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Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Chromatography: Introduction01:10

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

8.0K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Advances in cell membrane chromatography.

Weina Ma1, Cheng Wang1, Rui Liu1

  • 1School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China; Institute of Vascular Materia Medica, Xi'an Jiaotong University, Xi'an, Shaanxi 710116, China.

Journal of Chromatography. A
|February 6, 2021
PubMed
Summary
This summary is machine-generated.

Cell membrane chromatography (CMC) uses membrane receptors to identify drug targets and analyze interactions. This biomimetic method advances drug discovery and quality control by mimicking in vivo ligand-receptor binding.

Keywords:
Active ingredient screeningCell membrane chromatographyChinese medicineDrug–receptor interactionPseudo-allergy

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Cell membrane chromatography (CMC) is a biomimetic technique mirroring in vivo ligand-receptor interactions.
  • It utilizes membrane receptors as a stationary phase for selective binding analysis.

Purpose of the Study:

  • To determine binding characteristics between ligands and membrane receptors.
  • To identify specific target components in complex samples responsible for biological effects.
  • To serve as an analytical tool for drug-receptor interaction studies, target screening, and quality control.

Main Methods:

  • Immobilization of cell membrane receptors in vitro.
  • Development of various CMC models using different membrane receptors.
  • Integration of CMC with high-performance liquid chromatography (HPLC)/mass spectrometry and HPLC-IT-TOF.

Main Results:

  • Established CMC as a method for revealing ligand-receptor interaction characteristics.
  • Enabled efficient identification of specific target components in complex samples.
  • Facilitated accurate drug quality control through binding assays.

Conclusions:

  • CMC has significantly advanced due to progress in cell biology, receptor expression, and assay miniaturization.
  • Combined CMC techniques offer powerful analytical capabilities for drug development.
  • CMC is widely applied in studying drug-receptor interactions and screening for active or harmful components.