Related Experiment Video

Updated: Nov 18, 2025

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

4.0K

TRPV1 activation inhibits phenotypic switching and oxidative stress in vascular smooth muscle cells by upregulating

Yi Zhou1, Xueli Wang1, Lu Guo2

  • 1Department of Neurology, 980 Hospital of PLA Joint Logistics Support Forces, 398 ZhongShan Xi Road, QiaoXi District, ShiJiaZhuang, Hebei Province, China.

Biochemical and Biophysical Research Communications
|February 7, 2021
PubMed

Insights

Capsaicin-activated Transient Receptor Potential Vanilloid 1 (TRPV1) inhibits vascular smooth muscle cell (VSMC) proliferation and phenotypic switching. This occurs by reducing superoxide generation and upregulating peroxisome proliferator activated receptor α (PPARα), suggesting a therapeutic target for vascular remodeling.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Cellular Physiology

Background:

  • Vascular smooth muscle cell (VSMC) proliferation and migration drive vascular remodeling and diseases like atherosclerosis.
  • The role and regulatory mechanisms of Transient Receptor Potential Vanilloid 1 (TRPV1) in VSMC function remain unclear.

Purpose of the Study:

  • To investigate the effects of capsaicin-activated TRPV1 on VSMC phenotypic switching.
  • To elucidate the signaling pathways involved in TRPV1-mediated regulation of VSMCs.

Main Methods:

  • Oxidized low-density lipoprotein (ox-LDL) was used to induce VSMC proliferation and migration in vitro.
  • The impact of capsaicin-activated TRPV1 on superoxide anion generation and VSMC phenotypic switching was assessed.
  • The role of peroxisome proliferator activated receptor α (PPARα) in TRPV1 signaling was investigated.

Main Results:

  • Ox-LDL induced VSMC proliferation in a dose-dependent manner.
  • Capsaicin-activated TRPV1 significantly reduced ox-LDL-induced superoxide anion generation.
  • TRPV1 activation inhibited VSMC phenotypic switching, partly via upregulation of PPARα expression.
  • Capsaicin decreased ox-LDL-induced superoxide anion generation by activating PPARα.

Conclusions:

  • Capsaicin-activated TRPV1 inhibits VSMC phenotypic switching and proliferation.
  • TRPV1 exerts its effects by modulating superoxide anion generation and upregulating PPARα.
  • TRPV1 activation represents a potential therapeutic strategy for vascular remodeling and related diseases.
Keywords:
Oxidative stressPeroxisome proliferator activated receptor αPhenotypic switchingTransient receptor potential vanilloid 1

More Related Videos

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

39.2K
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice09:06

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Published on: February 20, 2019

8.6K

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
8.5K
Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
5.7K
Parasympathetic Signaling01:30

Parasympathetic Signaling

Parasympathetic signaling plays a crucial role in regulating various physiological processes. It involves the release of acetylcholine (ACh) by parasympathetic neurons, which can have localized and short-lived effects. The majority of ACh released is rapidly inactivated at the synapse by the enzyme acetylcholinesterase (AChE), which hydrolyzes Ach into choline and acetate. Additionally, the tissue cholinesterase deactivates any ACh diffusing into the surrounding tissues.
The effects of...
2.4K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.8K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.0K
Sympathetic Signaling01:31

Sympathetic Signaling

Sympathetic signaling, a vital part of the autonomic nervous system, plays a crucial role in mobilizing the body's resources in response to stress or emergencies. It involves the transmission of nerve impulses from sympathetic preganglionic fibers to postganglionic fibers. This results in the release of specific neurotransmitters and activation of adrenergic receptors.
Sympathetic preganglionic fibers release the neurotransmitter acetylcholine (ACh) onto the ganglionic neurons in the...
1.6K