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Abietane diterpenoids from Dracocephalum moldavica L. and their anti-inflammatory activities in vitro
Lanlan Nie1, Rui Li1, Jie Huang2
1State Key Lab. of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China.
Abstract:
Five undescribed abietane diterpenoids, dracocephalumoids A-E, together with six known analogues were isolated from the aqueous EtOH extract of aerial parts of Dracocephalum moldavica L.. The structures were elucidated through extensive analysis of spectroscopic data, and their absolute configurations were established by Mosher's method and time-dependent density functional theory electronic circular dichroism (TDDFT ECD) calculation. Cell-based anti-inflammatory assays displayed that dracocephalumoid A, uncinatone, trichotomone F and caryopterisoid C showed potent suppressive effects against TNF-α, IL-1β or NO production in LPS-induced RAW 264.7 cells, with IC50 values ranging from 1.12 to 5.84 μM. The structure-activity relationship of these compounds was also discussed.
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