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Chemiluminescence immunoassay for progesterone in plasma incorporating acridinium ester labelled antigen
S A Miller1, M S Morton, A Turkes
1Tenovus Institute for Cancer Research, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Abstract:
A sensitive, solid-phase chemiluminescence immunoassay suitable for determining progesterone concentrations in plasma has been developed. The solid-phase antiserum was prepared by coupling a monoclonal progesterone-antibody, raised against a progesterone-11 alpha-hemisuccinyl/bovine serum albumin conjugate, to cyanogen bromide activated cellulose. An 11 alpha-progesteryl-2-carboxymethyltyramine-4-(10-methyl)-a cri dinium-9-carboxylate conjugate was used as the chemiluminescent label. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Progesterone concentrations determined by chemiluminescence assay were in good agreement not only with a radioimmunoassay in routine use but also with a gas chromatography-mass spectrometry procedure.