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Updated: Nov 18, 2025
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
MicroRNA-144 Suppresses Prostate Cancer Growth and Metastasis by Targeting EZH2
Xin-Bo Sun1, Yong-Wei Chen1, Qi-Sheng Yao1
1Department of Urology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.
Background:
Prostate cancer is a common malignant tumor with a high incidence. MicroRNAs (miRNAs) have been shown to be important post-transcriptional regulators during tumorigenesis. This study aimed to explore the effect of miR-144 on PCa proliferation and apoptosis.
Material And Methods:
The expression of miR-144 and EZH2 were examined in clinical PCa tissues. PCa cell line LNCAP and DU-145 was employed and transfected with miR-144 mimics or inhibitors. The correlation between miR-144 and EZH2 was verified by luciferase reporter assay. Cell viability, apoptosis and migratory capacity were detected by CCK-8, flow cytometry assay and wound healing assay. The protein level of EZH2, E-Cadherin, N-Cadherin and vimentin were analyzed by western blotting.
Results:
miR-144 was found to be negatively correlated to the expression of EZH2 in PCa tissues. Further studies identified EZH2 as a direct target of miR-144. Moreover, overexpression of miR-144 downregulated expression of EZH2, reduced cell viability and promoted cell apoptosis, while knockdown of miR-144 led to an inverse result. miR-144 also suppressed epithelial-mesenchymal transition level of PCa cells.
Conclusion:
Our study indicated that miR-144 negatively regulate the expression of EZH2 in clinical specimens and in vitro. miR-144 can inhibit cell proliferation and induce cell apoptosis in PCa cells. Therefore, miR-144 has the potential to be used as a biomarker for predicting the progression of PCa.
Insights
MicroRNA-144 (miR-144) inhibits prostate cancer (PCa) cell growth and promotes apoptosis by downregulating EZH2. This suggests miR-144 is a potential biomarker for PCa progression.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Prostate cancer (PCa) is a prevalent malignancy.
- MicroRNAs (miRNAs) are key regulators in tumorigenesis.
- The role of miR-144 in PCa requires further investigation.
Purpose of the Study:
- To investigate the effect of miR-144 on PCa cell proliferation and apoptosis.
- To explore the relationship between miR-144 and EZH2 in PCa.
Main Methods:
- Examined miR-144 and EZH2 expression in PCa tissues and cell lines.
- Utilized miR-144 mimics/inhibitors and luciferase reporter assays.
- Assessed cell viability, apoptosis, migration, and epithelial-mesenchymal transition (EMT).
Main Results:
- miR-144 expression inversely correlated with EZH2 in PCa.
- EZH2 is a direct target of miR-144.
- miR-144 overexpression reduced cell viability and promoted apoptosis, while inhibiting EMT.
Conclusions:
- miR-144 negatively regulates EZH2 in PCa.
- miR-144 inhibits PCa cell proliferation and induces apoptosis.
- miR-144 shows potential as a biomarker for PCa progression.
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