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Comparative evaluation of apical extrusion of intracanal bacteria using ProTaper Next, Mtwo, and ProTaper rotary
Swapnika Polineni1, Bhargavi Damaraju2, Nagesh Bolla3
1Department of Dentistry, All India Institute of Medical Sciences, Mangalagiri, Andhra Pradesh, India.
Aim:
The aim of this study was to evaluate the number of intracanal bacteria extruded apically after instrumentation with three different nickel-titanium rotary instruments.
Materials And Methodology:
Forty freshly extracted mandibular premolars were selected, access cavities were prepared, and the teeth were mounted in the bacterial collection apparatus. Root canals were contaminated with a suspension of Enterococcus faecalis and incubated for 24 h at 37°C. The contaminated teeth were divided into four groups of 10 teeth each according to the rotary system used for instrumentation: Group 1: ProTaper universal files, Group 2: MTwo files, Group 3: ProTaper Next files, and Group 4: Control group (no instrumentation). Bacteria extruded after preparations were collected into vials. The number of colony-forming units (CFUs) was determined for each sample.
Statistical Analysis:
The data obtained were analyzed using the one-way analysis of variance followed by post hoc Tukey's test with a P = 0.05 as the level for statistical significance.
Results:
The results suggested a statistically significant difference in the number of CFUs between four experimental groups (P < 0.001).
Conclusion:
Least amount of bacterial extrusion was seen in ProTaper Next Group while more bacterial extrusion was seen in MTwo Group.
