Leucine transport system in a facultatively alkalophilic Bacillus

Some characterizations of the leucine transport system in a facultative alkalophile, which is able to grow over a wide pH range from 7.0 to 10.5, were attempted. Although the direction of a transmembrane pH gradient of the bacterium below pH 8.2 is opposite to that above pH 8.2 (N. Koyama and Y. Nosoh (1985) Biochim. Biophys. Acta 812, 206-212), leucine transport is likely to be driven only by sodium electrochemical potential irrespective of the external pH. It was suggested that histidine and sulfhydryl groups in the leucine transporter are involved in the translocation mechanism and the pK value of the histidine residue involved is approximately 7.0.