Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

726
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
726
Effect of Temperature Change on Reaction Rate02:28

Effect of Temperature Change on Reaction Rate

4.6K
The Arrhenius equation,
4.6K
Diversity of Archaea IV01:29

Diversity of Archaea IV

257
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
257
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

8.5K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
8.5K
Effects of Temperature on Free Energy02:11

Effects of Temperature on Free Energy

27.3K
The spontaneity of a process depends upon the temperature of the system. Phase transitions, for example, will proceed spontaneously in one direction or the other depending upon the temperature of the substance in question. Likewise, some chemical reactions can also exhibit temperature-dependent spontaneities. To illustrate this concept, the equation relating free energy change to the enthalpy and entropy changes for the process is considered:
27.3K
Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

22.9K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
22.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PET/CT imaging-based quantification of respiratory tract deposition of small- and large-particle aerosols versus intranasal bolus in rodents.

Inhalation toxicology·2026
Same author

Implementation and Use of Entrustable Professional Activities in the Internal Medicine Acting Internship.

Journal of general internal medicine·2026
Same author

Correction: Grant et al. Low pH, High Stakes: A Narrative Review Exploring the Acid-Sensing GPR65 Pathway as a Novel Approach in Renal Cell Carcinoma. <i>Cancers</i> 2025, <i>17</i>, 3883.

Cancers·2026
Same author

Consumption of ultra-processed foods and increased risks of cardiovascular disease in US adults.

The American journal of medicine·2026
Same author

Variations in Perinatal Interventions and Outcomes Among Active-Duty Service Women in the U.S. Military Health System.

Journal of women's health (2002)·2025
Same author

Anger-Related Affect and Suicidal Thoughts and Behaviors: A Systematic Review and Meta-Analysis.

Clinical psychology : a publication of the Division of Clinical Psychology of the American Psychological Association·2025

Related Experiment Video

Updated: Nov 18, 2025

A Tuberculosis Molecular Bacterial Load Assay TB-MBLA
10:41

A Tuberculosis Molecular Bacterial Load Assay TB-MBLA

Published on: April 30, 2020

14.5K

SARS-CoV-2 is rapidly inactivated at high temperature.

Jennifer Biryukov1, Jeremy A Boydston1, Rebecca A Dunning1

  • 1National Biodefense Analysis and Countermeasures Center (NBACC), Operated By Battelle National Biodefense Institute (BNBI) for the U.S. Department of Homeland Security Science and Technology Directorate, Fort Detrick, MD 21702 USA.

Environmental Chemistry Letters
|February 8, 2021
PubMed
Summary

High temperatures can inactivate the SARS-CoV-2 virus on surfaces. Studies show that at 54.5°C, the virus’s infectivity significantly decreases within an hour, reducing fomite transmission risk.

Keywords:
COVID-19CoronavirusEnvironmental decayHeatSARS-CoV-2Viral inactivation

More Related Videos

Determining Viral Disinfection Efficacy of Hot Water Laundering
06:57

Determining Viral Disinfection Efficacy of Hot Water Laundering

Published on: June 21, 2022

2.8K
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.0K

Related Experiment Videos

Last Updated: Nov 18, 2025

A Tuberculosis Molecular Bacterial Load Assay TB-MBLA
10:41

A Tuberculosis Molecular Bacterial Load Assay TB-MBLA

Published on: April 30, 2020

14.5K
Determining Viral Disinfection Efficacy of Hot Water Laundering
06:57

Determining Viral Disinfection Efficacy of Hot Water Laundering

Published on: June 21, 2022

2.8K
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.0K

Area of Science:

  • Virology
  • Environmental Science
  • Public Health

Background:

  • Preventing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread is crucial in the absence of a vaccine.
  • SARS-CoV-2 genetic material on surfaces suggests fomite transmission is possible.
  • High temperature's potential for virus inactivation requires further investigation.

Purpose of the Study:

  • To investigate the environmental stability and high-temperature inactivation of SARS-CoV-2.
  • To determine the virus's half-life and time to 90% infectivity decrease on stainless steel at elevated temperatures.

Main Methods:

  • Drying SARS-CoV-2 in a clinically relevant matrix onto stainless steel.
  • Incubating the contaminated surfaces at 54.5°C.
  • Measuring virus half-life and time for 90% infectivity reduction.

Main Results:

  • At 54.5°C, the virus half-life was 10.8 ± 3.0 minutes.
  • A 90% decrease in SARS-CoV-2 infectivity was observed within 35.4 ± 9.0 minutes.
  • High temperatures significantly reduce virus viability on surfaces.

Conclusions:

  • Temperatures of at least 54.5°C can substantially decrease SARS-CoV-2 infectivity on surfaces within an hour.
  • This finding has implications for reducing fomite transmission in environments that reach high temperatures, like vehicle interiors.
  • Environmental heat may serve as a viable disinfection method for SARS-CoV-2 contaminated surfaces.