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Enteric Phageome Alterations in Patients With Type 2 Diabetes

Qian Chen1, Xiaojing Ma2, Chong Li3

  • 1Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Frontiers in Cellular and Infection Microbiology
|February 8, 2021
PubMed

Insights

The gut phageome is altered in type 2 diabetes (T2D), with increased phages targeting Enterobacteriaceae bacteria. This phageome shift may play a role in T2D development.

Area of Science:

  • Microbiome research
  • Virology
  • Metabolic disease research

Background:

  • Type 2 diabetes (T2D) involves gut microbiota alterations, but the phageome's role is understudied.
  • Phages, viruses infecting bacteria, can modulate the gut bacterial community.
  • Investigating the phageome offers new insights into T2D pathogenesis.

Purpose of the Study:

  • To investigate changes in the extracellular phageome in type 2 diabetes patients.
  • To explore the phageome's role in T2D pathogenesis.
  • To identify potential phage-based biomarkers for T2D.

Main Methods:

  • Isolation of virus-like particles (VLPs) from fecal samples.
  • Sequencing-based identification of bacteriophages and their predicted hosts.
  • Comparison of phageome composition between T2D patients and nondiabetic controls.

Main Results:

  • Identified 330 phage species; phageome composition was highly individual.
  • T2D patients showed altered phage populations, with increased abundance of phages targeting Enterobacteriaceae.
  • Enterobacteriaceae and systemic LPS levels were elevated in T2D patients.
  • A specific eight-phage consortium accurately distinguished T2D patients (AUC>0.99).

Conclusions:

  • The gut phageome is significantly altered in type 2 diabetes.
  • Increased abundance of Enterobacteriaceae-specific phages correlates with increased Enterobacteriaceae and LPS levels in T2D.
  • The identified phage consortium shows potential as a diagnostic biomarker for T2D.
Keywords:
bacterial microbiomebacteriophagelipopolysaccharidephageometype 2 diabetes

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