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Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue.

M Sadman Sakib¹, Godwin Sokpor², Huu Phuc Nguyen²

¹German Center for Neurodegenerative Diseases, 37077 Goettingen, Germany.

|February 8, 2021

Summary

This summary is machine-generated.

This study introduces an improved intranuclear immunostaining method for fluorescence-activated cell sorting (FACS), enabling better purification of basal intermediate progenitors from embryonic brain tissue for molecular analysis.

Keywords:

Flow Cytometry/Mass Cytometry Genomics Neuroscience

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Area of Science:

Neuroscience
Cell Biology
Genomics

Background:

Cell sorting is crucial for isolating specific cell populations for molecular studies.
Fluorescence-activated cell sorting (FACS) combined with high-throughput sequencing aids in identifying cell-specific molecular signatures.
Current FACS methods face challenges in comprehensively purifying brain cells, limiting detailed molecular characterization.

Purpose of the Study:

To present an optimized intranuclear immunostaining-based FACS protocol.
To enable enhanced purification of specific cell types from embryonic cortical tissue.
To facilitate downstream molecular investigation of basal intermediate progenitors.

Main Methods:

Development of a modified intranuclear immunostaining protocol for FACS.
Application of the protocol to mouse and human embryonic cortical tissue.
Optimization of nuclei/cell sorting for downstream molecular analysis.

Main Results:

The developed protocol allows for optimized sorting of nuclei/cells from embryonic cortical samples.
This method addresses limitations of standard FACS in brain cell purification.
Enables distinct downstream molecular investigation of specific progenitor populations.

Conclusions:

The intranuclear immunostaining-based FACS protocol enhances cell purification efficiency from embryonic brain tissue.
This optimized method supports comprehensive molecular characterization of basal intermediate progenitors.
The protocol is applicable to both mouse and human embryonic cortical samples.