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Genomic DNA in Prokaryotes

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The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
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Comparative genomic analyses of Inc_pA1763-KPC plasmids.

Hongchao Chen¹, Zhe Zhan², Xiaoyuan Jiang³

¹College of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China.

Journal of Basic Microbiology

|February 8, 2021

Summary

This summary is machine-generated.

This study reveals that single Inc_pA1763-KPC plasmids, though poorly studied, are evolving. Comparative genomics shows their backbone structures vary, with accessory modules driving diversification.

Keywords:

IncpA1763-KPC plasmids Klebsiella pneumonia antibiotic resistance mobile elements

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Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Multi-replicon plasmids with Inc_pA1763-KPC are increasingly reported in Enterobacteriaceae.
Single Inc_pA1763-KPC plasmids are less understood despite their growing prevalence.

Purpose of the Study:

To investigate the genomic structure and evolution of single Inc_pA1763-KPC plasmids.
To compare the backbone regions of Inc_pA1763-KPC plasmids from clinical isolates with previously sequenced plasmids.

Main Methods:

High-throughput genome sequencing of two clinical Klebsiella pneumoniae isolates.
Linear structural and detailed genomic comparisons of Inc_pA1763-KPC plasmids.
Analysis of backbone regions, maintenance regions, and accessory modules.

Main Results:

Plasmids pA1763-KPC and p427113-2 possess complete Inc_pA1763-KPC backbones.
Other Inc_pA1763-KPC plasmids exhibit deletions or truncations in their backbone regions.
Accessory modules, including resistance genes (bla_KPC-2, ars, ncr, sil) and insertion sequences, are integrated into plasmid backbones.

Conclusions:

Comparative genomics provides insight into the diversification and evolution of Inc_pA1763-KPC plasmids.
Variations in backbone structure and accessory module integration contribute to plasmid evolution.