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Comparative genomic analyses of IncpA1763-KPC plasmids
Hongchao Chen1, Zhe Zhan2, Xiaoyuan Jiang3
1College of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China.
Journal of Basic Microbiology
|February 8, 2021
Summary
This study reveals that single IncpA1763-KPC plasmids, though poorly studied, are evolving. Comparative genomics shows their backbone structures vary, with accessory modules driving diversification.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Multi-replicon plasmids with IncpA1763-KPC are increasingly reported in Enterobacteriaceae.
- Single IncpA1763-KPC plasmids are less understood despite their growing prevalence.
Purpose of the Study:
- To investigate the genomic structure and evolution of single IncpA1763-KPC plasmids.
- To compare the backbone regions of IncpA1763-KPC plasmids from clinical isolates with previously sequenced plasmids.
Main Methods:
- High-throughput genome sequencing of two clinical Klebsiella pneumoniae isolates.
- Linear structural and detailed genomic comparisons of IncpA1763-KPC plasmids.
- Analysis of backbone regions, maintenance regions, and accessory modules.
Main Results:
- Plasmids pA1763-KPC and p427113-2 possess complete IncpA1763-KPC backbones.
- Other IncpA1763-KPC plasmids exhibit deletions or truncations in their backbone regions.
- Accessory modules, including resistance genes (blaKPC-2, ars, ncr, sil) and insertion sequences, are integrated into plasmid backbones.
Conclusions:
- Comparative genomics provides insight into the diversification and evolution of IncpA1763-KPC plasmids.
- Variations in backbone structure and accessory module integration contribute to plasmid evolution.
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