Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Light mitochondria and cellular thermogenesis.

F Goglia1, G Liverini, A Lanni

  • 1Department of General and Environmental Physiology, University of Naples, Italy.

Biochemical and Biophysical Research Communications
|March 30, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anomalous mass diffusion in a binary mixture and Rayleigh-Bénard instability.

Physical review. E·2024
Same author

Decreasing trend of drug-resistant TB in Italy.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2022
Same author

Cerebral superb microvascular imaging in preterm neonates: in vivo evaluation of thalamic, striatal, and extrastriatal angioarchitecture.

Neuroradiology·2021
Same author

The Horton-Rogers-Lapwood problem for an inclined porous layer with permeable boundaries.

Proceedings. Mathematical, physical, and engineering sciences·2018
Same author

Beneficial effects of carotenoid-producing cells of Bacillus indicus HU16 in a rat model of diet-induced metabolic syndrome.

Beneficial microbes·2017
Same author

Both 3,5-diiodo-L-thyronine (T2) and T3 modulate glucose-induced insulin secretion.

Journal of biological regulators and homeostatic agents·2017

Mitochondria subpopulations show distinct energy production strategies. Smaller mitochondria optimize heat, crucial for cellular thermogenesis regulation, especially after cold exposure.

Area of Science:

  • Mitochondrial biology
  • Cellular metabolism
  • Thermogenesis

Background:

  • Mitochondria are key to cellular energy production through oxidative phosphorylation.
  • Understanding mitochondrial subpopulations is vital for metabolic research.
  • Cellular thermogenesis is critical for maintaining body temperature.

Purpose of the Study:

  • To investigate differences in oxidative phosphorylation coupling among mitochondrial subpopulations.
  • To explore the role of mitochondrial subpopulations in cellular thermogenesis.
  • To analyze the impact of cold exposure on mitochondrial function.

Main Methods:

  • Separation of mitochondrial subpopulations by differential centrifugation (3,000 g, 10,000 g, 27,000 g).
  • Assessment of ATP synthesis and heat production in each fraction.

Related Experiment Videos

  • Measurement of oxygen consumption following cold exposure.

    • Main Results:

    • Significant variations in oxidation-phosphorylation coupling were observed across three mitochondrial fractions.
    • Heavy fraction (3,000 g) optimized ATP synthesis.
    • Medium and light fractions (10,000 g, 27,000 g) showed optimized heat production with decreasing mitochondrial size.
    • Light fraction oxygen consumption increased by 150% after 15 days of cold exposure.

    Conclusions:

    • Mitochondrial subpopulations exhibit specialized roles in energy metabolism.
    • Decreasing mitochondrial size correlates with optimized heat production, suggesting a role in thermogenesis.
    • Cold exposure significantly impacts mitochondrial function, particularly in smaller subpopulations, highlighting their importance in cellular thermogenesis regulation.