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Updated: Nov 18, 2025
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Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
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SUV39H2 controls trophoblast stem cell fate
Lei Wang1, Damayanti Chakraborty1, Khursheed Iqbal1
1Institute for Reproduction and Perinatal Research, Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, United States of America.
Biochimica Et Biophysica Acta. General Subjects
|February 8, 2021
Summary
Suppressor of variegation 3-9 homolog 2 (SUV39H2) maintains trophoblast stem cells and restrains their differentiation. This epigenetic regulator is crucial for placental development.
Area of Science:
- Developmental Biology
- Epigenetics
- Stem Cell Biology
Background:
- Trophoblast stem (TS) cells differentiate to form the placenta.
- Histone 3 lysine 9 (H3K9) methylation dynamics are vital for cell differentiation.
- The role of H3K9 methylation in trophoblast development is largely unknown.
Purpose of the Study:
- Investigate the role of H3K9 methyltransferases in rat TS cell differentiation.
- Determine the specific function of suppressor of variegation 3-9 homolog 2 (SUV39H2) in trophoblast development.
Main Methods:
- Surveyed H3K9 methyltransferase expression in rat TS cells.
- Utilized a loss-of-function approach for SUV39H2 in TS cells and ex vivo blastocysts.
Main Results:
- SUV39H2 showed differential expression, high in stem cells and declining upon differentiation.
- SUV39H2 disruption arrested TS cell proliferation and triggered differentiation.
- SUV39H2 regulates H3K9 methylation at differentiation-dependent loci.
- SUV39H2 is a downstream target of caudal type homeobox 2.
Conclusions:
- SUV39H2 maintains TS cell identity.
- SUV39H2 restrains trophoblast cell differentiation.
- SUV39H2 is key to epigenetic regulation in placental development.
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