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Updated: Nov 18, 2025
10:14
Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
Published on: October 25, 2014
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Physiological interrelationships between NADPH oxidases and chromatin remodelling.
1School of Cardiovascular Medicine & Sciences, King's College London British Heart Foundation Centre of Research Excellence, London, UK.
Free Radical Biology & Medicine
|February 8, 2021
Summary
Reactive oxygen species (ROS) generated by NADPH oxidases regulate epigenetic changes. This review explores how these enzymes impact chromatin remodelling and gene expression during cellular differentiation and homeostasis.
Area of Science:
- Molecular Biology
- Epigenetics
- Cellular Biology
Background:
- The epigenetic landscape, or chromatin structure, dictates gene transcription and cellular phenotype.
- Dynamic chromatin remodelling is crucial for cellular differentiation and adaptation to stress.
- Redox-dependent mechanisms, particularly reactive oxygen species (ROS), are increasingly recognized as key epigenetic regulators.
Purpose of the Study:
- To review the role of NADPH oxidases in redox-dependent chromatin remodelling.
- To explore the feedback mechanisms between epigenetic changes and NADPH oxidase gene expression.
- To discuss the physiological significance of Nox4 in cellular differentiation and homeostasis.
Main Methods:
- Literature review of studies on NADPH oxidases, ROS, and epigenetics.
- Analysis of molecular mechanisms linking redox signalling to chromatin modification.
- Synthesis of current knowledge on the interplay between epigenetics and NADPH oxidase function.
Main Results:
- NADPH oxidases generate ROS that modulate redox-dependent signalling pathways.
- ROS influence chromatin structure, impacting gene transcription.
- Epigenetic modifications can, in turn, affect the expression of NADPH oxidase enzymes.
Conclusions:
- NADPH oxidases are critical mediators of redox-dependent epigenetic regulation.
- The interplay between ROS and epigenetics is vital for cellular differentiation and homeostasis.
- Nox4 plays a significant role in maintaining cellular physiological balance through epigenetic control.
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