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Morphometric study of bone marrow in chronic lymphocytic leukemia
E Lerma-Puertas1, R González-Cámpora, J Amérigo-Navarro
1Department of Pathology, University of Seville, Spain.
Analytical and Quantitative Cytology and Histology
|February 1, 1988
Summary
This study found that higher lymphocytic infiltration in chronic lymphocytic leukemia (CLL) bone marrow correlates with interstitial/diffuse patterns and stages A/C. However, this infiltration level did not significantly impact survival rates after four years.
Area of Science:
- Hematology
- Oncology
- Pathology
Background:
- Chronic lymphocytic leukemia (CLL) is a heterogeneous lymphoid malignancy.
- Histologic patterns and tumor burden are key factors in CLL prognosis.
- Accurate morphometric assessment of bone marrow infiltration is crucial for understanding disease progression.
Purpose of the Study:
- To quantitatively correlate lymphocytic infiltration volume in bone marrow with histologic patterns and clinical stages in CLL patients.
- To evaluate the prognostic significance of tumor cell burden in CLL.
- To investigate the relationship between lymphoid tissue volume and patient survival.
Main Methods:
- A morphometric study using the point counting method on bone marrow biopsies from 44 CLL patients.
- Correlation analysis between objective volume of lymphocytic infiltration (VL) and histologic patterns (nodular, interstitial, mixed, diffuse).
- Correlation analysis between VL and clinical stages (A, B, C) according to Rai staging system.
Main Results:
- Significant correlation observed between high lymphoid tissue volume (VL > 60%) and interstitial/diffuse histologic patterns.
- Significant correlation found between high lymphoid tissue volume (VL > 60%) and clinical stages A and C.
- No significant correlation was found between lymphoid burden and nodular/mixed patterns, or clinical stage B.
- Cumulative survival rates did not differ significantly between patients with VL > 60% and VL < 60% after four years.
Conclusions:
- Objective volume of lymphocytic infiltration in CLL bone marrow is significantly associated with specific histologic patterns and clinical stages.
- While tumor burden correlates with certain patterns and stages, it did not demonstrate a significant impact on long-term survival in this cohort.
- Morphometric analysis provides valuable insights into CLL heterogeneity and its relationship with disease characteristics.