Circulating microRNA-126 &122 in patients with coronary artery disease: Correlation with small dense LDL
Sridhar Mishra1, Aliya Rizvi1, Akshay Pradhan2
1Department of Pathology, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
Insights
MicroRNA-126 (miR-126) levels were not significantly different in coronary artery disease (CAD) patients overall, but decreased with high small dense low-density lipoprotein (sdLDL). MiR-126 may play a role in sdLDL cholesterol metabolism.
Area of Science:
- Cardiovascular Diseases
- Molecular Biology
- Biochemistry
Background:
- Coronary artery disease (CAD) and stroke are leading causes of cardiovascular death.
- Conventional risk factors inadequately explain atherosclerosis progression, necessitating new diagnostic markers.
- MicroRNAs (miRNAs) regulate gene expression in cardiovascular disease pathogenesis, with endothelial dysfunction being an early event in atherosclerosis.
Purpose of the Study:
- To investigate alterations in vascular endothelium-enriched microRNAs in patients with coronary artery disease (CAD).
Main Methods:
- Serum levels of miR-126 and miR-122 were quantified using qRT-PCR in 78 CAD patients and 60 non-CAD controls.
- Analysis correlated miRNA levels with CAD status and small dense low-density lipoprotein (sdLDL) levels.
Main Results:
- miR-122 was significantly downregulated in CAD patients (p = 0.001).
- miR-126 levels did not differ significantly between CAD and non-CAD patients (p = 0.507).
- miR-126 was significantly decreased in CAD patients with high sdLDL and increased in those with risk factors but no significant CAD when sdLDL was elevated.
Conclusions:
- miR-126 shows a potential role in small dense low-density lipoprotein (sdLDL) cholesterol metabolism, despite no significant overall difference in CAD patients.
- miR-122 is downregulated in CAD, suggesting its involvement in cardiovascular disease processes like inflammation and cholesterol biosynthesis.
Background:
Coronary artery disease (CAD) and stroke are major causes of cardiovascular diseases related deaths. Conventional risk factors cannot explain the changes in atherosclerosis. New and useful diagnostic markers are required. MicroRNAs are small, noncoding RNA that regulate the gene expression implicated in the pathogenesis of various cardiovascular diseases. Endothelial dysfunction is involved in the early event of the atherosclerosis process.
Aims:
The current study was designed to evaluate the vascular endothelium-enriched miRNAs would be altered in CAD patients.
Methods:
Circulating miR-126 & 122 levels were measured in serum from 78 CAD patients and 60 non CDA patients by qRT-PCR analysis.
Results:
MiR-122 was significantly down regulated in CAD patients (p = 0.001), however the level of miR-126 did not show any change (p = 0.507). Remarkably, the level of miR-126 was significantly decreased in patients with CAD and high small dense low density lipoprotein (sdLDL) level. The level of miR-126 was significantly increased when sdLDL was higher in patients with risk factors for CAD but did not have angiographically significant CAD.
Conclusion:
. In CAD patient's, miR-126 level was lowered compared to non CAD patients, however the difference was not significant (0.507). However we found a direct relationship between endothelium-enriched miR-126 and sdLDL in patients with or without CAD. Our finding suggests that miR-126 may have a potential role in sdLDL cholesterol metabolism. Mir-122 plays a role in cholesterol biosynthesis and deteriorates the cardiovascular system through the process of inflammation, apoptosis, oxidative stress and ECM deposition in a number of cardiovascular diseases.
More Related Videos
Related Concept Videos
01:22MicroRNAs
01:22MicroRNAs
01:26Coronary Artery Disease II: Pathophysiology
01:26Coronary Artery Disease IV: Preventive Measures