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Updated: Nov 18, 2025

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
Usage considerations for headspace-gas chromatography-ion mobility spectrometry as a suitable technique for
Natividad Jurado-Campos1, Andrés Martín-Gómez1, David Saavedra1
1Department of Analytical Chemistry, University of Córdoba, Faculty of Sciences, International Agri-food Campus of Excellence (ceiA3), Marie Curie Annex Building, Campus de Rabanales, 14071 Córdoba, Spain.
Abstract:
The potential of headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) to perform non-targeted qualitative analysis of complex samples has led to an unprecedented increase in its popularity in recent years. The operating principle of IMS makes quality control essential to ensure adequate results. Besides this, the suitability of GC-IMS is determined by multiple phenomena that take place before and during IMS detection. The present work discusses a novel GC-IMS quality control protocol for both beginners and experienced users. Likewise, it describes factors that must be taken into account in order to develop a robust GC-IMS qualitative analysis method and, if needed, to achieve the identification of VOCs present in real samples. The developed quality control protocol was successfully employed in our laboratory for the routine analysis of >500 real samples (olive oil and Iberian ham) for 6 months, thus it is recommended for the analysis of a great number of complex samples. Furthermore, the behaviour of the ions produced in the ionisation chamber and the possible reactions between them in GC-IMS qualitative analysis were assessed.
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