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Recombinant production of poly-(3-hydroxybutyrate) by Bacillus megaterium utilizing millet bran and rapeseed meal
Subbi Rami Reddy Tadi1, Som Dutt Ravindran2, Rengesh Balakrishnan3
1BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Abstract:
Fermentative poly-3-hydroxybutyrate (PHB) production is mainly limited by the cost of raw material. In this present study, low-cost feedstock viz., millet bran residue (MBRH) and rapeseed meal hydrolysates were successfully utilized for PHB production. Metabolic engineering of Bacillus megaterium by co-expression of both precursor (phbRBC) and NADPH cofactor regeneration (zwf) genes resulted in 2.67-fold enhancement in PHB accumulation compared to wild strain. Modified logistic model characterized B.megaterium growth and PHB production effectively. The kinetic analysis proved that maximum cell concentration (15.01 g.L-1) and growth-associated constant (0.22 g.g-1) were found to be higher for initial MBRH concentration (S0 = 20 g.L-1). PHB production kinetics elucidated its expression in B.megaterium was growth-associated. PHB synthesized by B.megaterium was characterized using FTIR, NMR, XRD, DSC/TGA, FESEM and the physio-chemical properties enumerated its as a potential biodegradable plastic for industrial application.
