Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phylogenomics reveals multiple evolutionary lineages of marine crustacean-infecting gregarine apicomplexans.

Scientific reports·2026
Same author

Single-cell phylogenomics identifies major groups of marine eugregarine endosymbionts (Apicomplexa).

Molecular phylogenetics and evolution·2026
Same author

Divergent Plastid Genomes in the Deepest-Branching Apicomplexan Parasites.

Genome biology and evolution·2025
Same author

X-ray photoelectron spectroscopy of metal oxide nanoparticles: chemical composition, oxidation state and functional group content.

Nanoscale advances·2025
Same author

Titanium dioxide nanoparticles - physicochemical characterization and cytotoxic risk.

NanoImpact·2025
Same author

Phylogenomic diversity of archigregarine apicomplexans.

Open biology·2024

Related Experiment Video

Updated: Nov 18, 2025

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria
08:22

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria

Published on: May 16, 2025

389

Size-Specific Copper Nanoparticle Cytotoxicity Varies between Human Cell Lines.

Ina Na1, David C Kennedy1

  • 1Metrology, National Research Council Canada, 1200 Montreal Road, Ottawa, ON K1A 0R6, Canada.

International Journal of Molecular Sciences
|February 9, 2021
PubMed
Summary

Copper nanoparticles (CuNPs) show size-dependent cytotoxicity. Intermediate-sized CuNPs (40-60 nm) were most toxic, correlating with higher cellular uptake and instability in cell culture media.

Keywords:
copper nanoparticlecytotoxicityspectroscopystabilityuptake

More Related Videos

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability
09:23

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Published on: June 21, 2015

10.0K
A New Portable In Vitro Exposure Cassette for Aerosol Sampling
07:01

A New Portable In Vitro Exposure Cassette for Aerosol Sampling

Published on: February 22, 2019

7.4K

Related Experiment Videos

Last Updated: Nov 18, 2025

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria
08:22

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria

Published on: May 16, 2025

389
Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability
09:23

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Published on: June 21, 2015

10.0K
A New Portable In Vitro Exposure Cassette for Aerosol Sampling
07:01

A New Portable In Vitro Exposure Cassette for Aerosol Sampling

Published on: February 22, 2019

7.4K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Toxicology

Background:

  • Copper nanoparticles (CuNPs) are increasingly used in various applications.
  • Understanding their toxicological profiles is crucial for safe application.
  • Particle properties like size, stability, and cellular uptake influence cytotoxicity.

Purpose of the Study:

  • To investigate the cytotoxicity of different sized copper nanoparticles.
  • To correlate cytotoxicity with particle stability and cellular uptake.
  • To elucidate size-dependent trends in copper nanoparticle toxicity.

Main Methods:

  • Tested commercially available CuNPs of three sizes against three human cell lines.
  • Employed four distinct cytotoxicity assays.
  • Monitored particle stability and cellular uptake over time.

Main Results:

  • CuNPs exhibited instability in cell culture media, affecting assay results.
  • Intermediate-sized CuNPs (40-60 nm) demonstrated higher cytotoxicity.
  • Higher cellular uptake of intermediate-sized CuNPs correlated with increased toxicity.
  • Unique stability dynamics were observed for intermediate-sized particles.

Conclusions:

  • Copper nanoparticle cytotoxicity is strongly influenced by particle size and stability in biological media.
  • Intermediate-sized CuNPs present a distinct toxicological profile due to enhanced uptake and altered stability.
  • Further research is needed to understand the specific mechanisms behind the unique properties of intermediate-sized CuNPs.