Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein and Protein Structure02:15

Protein and Protein Structure

84.6K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
84.6K
Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

46.0K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
46.0K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.2K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.2K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

85.5K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
85.5K
Protein Glycosylation01:25

Protein Glycosylation

8.4K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
8.4K
Structural Protein Function01:56

Structural Protein Function

29.3K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
29.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Branching of O-mannose glycans regulates node of Ranvier organization and saltatory conduction.

Communications biology·2026
Same author

Prediction of Novel Disease-Related Regions in SIGLEC-7 by <i>In Silico</i> and Biochemical Analyses.

International journal of molecular sciences·2026
Same author

Pairing of high-mannose glycans in human IgE-Fc: implications for secretion and stability.

Clinical proteomics·2026
Same author

Integrative structural analysis of lacto-N-fucopentaose I recognition by antibodies R-17F and R-13E using NMR, docking, and molecular dynamics simulations.

Glycobiology·2026
Same author

Structural determinants of site-specific Core Fucosylation in human transferrin: insights from crystallography and molecular dynamics.

Glycobiology·2026
Same author

Monoclonal antibody-based assay to identify compounds that inhibit N<sup>δ</sup>-(5-hydro-4-imidazolon-2-yl)ornithine (G-H1) formation.

Glycoconjugate journal·2025

Related Experiment Video

Updated: Nov 18, 2025

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
09:07

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Published on: December 23, 2022

1.6K

3D Structural Insights into β-Glucans and Their Binding Proteins.

Noriyoshi Manabe1, Yoshiki Yamaguchi1

  • 1Division of Pharmaceutical Physical Chemistry, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan.

International Journal of Molecular Sciences
|February 9, 2021
PubMed
Summary

Beta-glucans, found in fungi and plants, are key to host defense. This review explores their 3D structures and how these impact interactions with beta-glucan-binding proteins.

Keywords:
3D structureDectin-1NMRX-ray crystallographyendoglucanasetriple helixβ-glucanβGRP/GNBP3

More Related Videos

Isolation and Purification of Fungal &#946;-Glucan as an Immunotherapy Strategy for Glioblastoma
10:02

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

2.2K
Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method
06:13

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method

Published on: March 31, 2022

3.9K

Related Experiment Videos

Last Updated: Nov 18, 2025

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
09:07

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Published on: December 23, 2022

1.6K
Isolation and Purification of Fungal &#946;-Glucan as an Immunotherapy Strategy for Glioblastoma
10:02

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

2.2K
Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method
06:13

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method

Published on: March 31, 2022

3.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Beta-glucans are essential structural polysaccharides in fungal and plant cell walls.
  • Pathogen-derived beta-glucans are recognized by host defense systems as foreign molecules.
  • The triple helix structure of long beta-glucan chains influences interactions with binding proteins.

Purpose of the Study:

  • To review the three-dimensional (3D) structural characteristics of beta-glucans.
  • To elucidate the modes of interaction between beta-glucans and beta-glucan-binding proteins.

Main Methods:

  • Review of existing literature.
  • Analysis of data from X-ray crystallography and NMR spectroscopy.
  • Examination of structural aspects of beta-glucan-binding protein interactions.

Main Results:

  • Beta-glucans exhibit diverse 3D structures, including triple helix formations.
  • Specific structural features of beta-glucans dictate their binding affinities and specificities.
  • Understanding these interactions is crucial for deciphering host-pathogen recognition pathways.

Conclusions:

  • The 3D structure of beta-glucans is a critical determinant of their biological function and interaction with host proteins.
  • Further structural studies are needed to fully understand beta-glucan recognition and signaling in host defense.