Related Experiment Videos
The turnover of lysosomal glycosylasparaginase in rat liver
O K Tollersrud1, S H Hofmann, N N Aronson
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park.
Biochimica Et Biophysica Acta
|April 14, 1988
Abstract:
Rat liver glycosylasparaginase (N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase (EC 3.5.1.26) was irreversibly inhibited in vitro by the asparagine analogue 5-diazo-4-oxo-L-norvaline (DONV). Following the abolition of this lysosomal hydrolase by DONV in rats, the enzyme activity in liver recovered exponentially. Assuming a zero order rate of protein synthesis, the half-life of the glycosylasparaginase in rat liver was measured to be 2 days.