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Related Experiment Videos

Immunophenotypic differences between plasmacytoma/multiple myeloma and immunoblastic lymphoma.

J G Strickler1, M W Audeh, C M Copenhaver

  • 1Department of Pathology, Stanford University Medical Center, CA 94305.

Cancer
|May 1, 1988
PubMed
Summary

Immunophenotypic analysis differentiated plasmacytoma/multiple myeloma from immunoblastic lymphoma using antibody panels. Proliferation marker Ki-67 correlated with prognosis in these B-cell malignancies.

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Area of Science:

  • Hematology
  • Immunology
  • Oncology

Background:

  • Plasmacytoma (PC) and multiple myeloma (MM) are plasma cell neoplasms, while immunoblastic lymphoma (IL) is a B-cell lymphoma.
  • Distinguishing between PC/MM and IL can be challenging based on morphology alone.

Purpose of the Study:

  • To investigate the immunophenotypic differences between PC/MM and IL using a panel of monoclonal antibodies.
  • To assess the utility of Ki-67 as a proliferation marker in relation to prognosis.

Main Methods:

  • Analysis of frozen tissue sections from four PC, six MM, and nine IL cases.
  • Application of a large panel of monoclonal antibodies, including lineage-specific and proliferation markers (Ki-67).

Main Results:

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  • PC/MM cases showed characteristic staining with alpha/gamma and T10, and lacked staining for mu, leukocyte common antigen, B-cell markers (B1, T015, 4G7, 6A4), and Ia.
  • IL cases typically stained positive for mu/gamma, leukocyte common antigen, B-cell markers, and Ia, but negative for alpha and T10.
  • Ki-67 staining was significantly lower in PC/MM compared to IL and inversely correlated with favorable clinical outcomes.

    • Conclusions:

    • A panel of monoclonal antibodies can aid in distinguishing PC/MM from IL.
    • Ki-67 reactivity may serve as a prognostic indicator in these B-cell malignancies.