Related Experiment Video
Updated: Nov 18, 2025
08:07
A Morphometric and Cellular Analysis Method for the Murine Mandibular Condyle
Published on: January 11, 2018
8.6K
ADAMTS5 is required for normal trabeculated bone development in the mandibular condyle
A W Rogers-DeCotes1, S C Porto2, L E Dupuis3
1Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, 29525, USA.
Osteoarthritis and Cartilage
|February 9, 2021
Summary
The extracellular matrix protease ADAMTS5 is crucial for mandibular condyle bone development. Its absence leads to altered bone structure and reduced osteoblast formation, suggesting a role in chondrocyte-to-osteoblast differentiation.
Area of Science:
- Skeletal Biology
- Temporomandibular Joint Development
- Extracellular Matrix Biology
Background:
- The mandibular condyle's trabeculated bone formation is essential for temporomandibular joint function.
- The role of specific extracellular matrix proteases in this developmental process remains largely unexplored.
Purpose of the Study:
- To investigate the function of ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5) in the development of trabeculated bone within the mandibular condyle.
Main Methods:
- Histopathological analysis of mandibular condyles from wild-type and Adamts5-deficient mice using Safranin O staining.
- Microcomputed tomography (micro-CT) for quantitative analysis of bone structure.
- RNAscope and immunohistochemistry to assess gene and protein expression in specific cell types.
Main Results:
- Adamts5-deficient mice exhibited increased trabecular separation and reduced trabecular thickness and bone volume fraction in the mandibular condyle.
- These bone alterations were more pronounced in male mice.
- Loss of Adamts5 led to decreased expression of osteoblast markers (Bglap) and reduced levels of the mineralization-associated protease Mmp13.
Conclusions:
- This study identifies a novel and critical role for ADAMTS5 in mandibular condyle bone formation.
- ADAMTS5 is implicated in the transdifferentiation of hypertrophic chondrocytes to osteoblasts during mandibular condyle development.
Related Concept Videos
01:29Bone Formation by Intramembranous Ossification
9.2K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
9.2K
01:24Bone Formation by Endochondral Ossification
7.3K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
7.3K
01:27Growth of Cartilage and Bone Tissue
3.8K
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
3.8K
01:18The Bone Matrix
5.1K
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
5.1K
01:31Essential Minerals for Bone Health
5.4K
The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
5.4K
01:40Bone Remodeling
39.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
39.4K