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Updated: Nov 18, 2025

A Morphometric and Cellular Analysis Method for the Murine Mandibular Condyle
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ADAMTS5 is required for normal trabeculated bone development in the mandibular condyle.

A W Rogers-DeCotes1, S C Porto2, L E Dupuis3

  • 1Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, 29525, USA.

Osteoarthritis and Cartilage
|February 9, 2021
PubMed
Summary

The extracellular matrix protease ADAMTS5 is crucial for mandibular condyle bone development. Its absence leads to altered bone structure and reduced osteoblast formation, suggesting a role in chondrocyte-to-osteoblast differentiation.

Keywords:
ADAMTSBoneExtracellular matrixMandibular condyleTemporomandibular joint (TMJ)

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Area of Science:

  • Skeletal Biology
  • Temporomandibular Joint Development
  • Extracellular Matrix Biology

Background:

  • The mandibular condyle's trabeculated bone formation is essential for temporomandibular joint function.
  • The role of specific extracellular matrix proteases in this developmental process remains largely unexplored.

Purpose of the Study:

  • To investigate the function of ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5) in the development of trabeculated bone within the mandibular condyle.

Main Methods:

  • Histopathological analysis of mandibular condyles from wild-type and Adamts5-deficient mice using Safranin O staining.
  • Microcomputed tomography (micro-CT) for quantitative analysis of bone structure.
  • RNAscope and immunohistochemistry to assess gene and protein expression in specific cell types.

Main Results:

  • Adamts5-deficient mice exhibited increased trabecular separation and reduced trabecular thickness and bone volume fraction in the mandibular condyle.
  • These bone alterations were more pronounced in male mice.
  • Loss of Adamts5 led to decreased expression of osteoblast markers (Bglap) and reduced levels of the mineralization-associated protease Mmp13.

Conclusions:

  • This study identifies a novel and critical role for ADAMTS5 in mandibular condyle bone formation.
  • ADAMTS5 is implicated in the transdifferentiation of hypertrophic chondrocytes to osteoblasts during mandibular condyle development.