BnaA02.NIP6;1a encodes a boron transporter required for plant development under boron deficiency in Brassica napus

Boron (B) is an essential micronutrient for the plant normal growth. In Arabidopsis, NIP6;1 is a boric acid channel required for the proper distribution of boric acid, especially in the nodal regions of shoots. BnaA02.NIP6;1a, a homologous gene of AtNIP6;1 in Brassica napus, was reported to play a key role in B transport activity. However, little is known about the other functions of BnaA02.NIP6;1a in Brassica napus. In this study, we found that BnaA02.NIP6; 1a was localized in both plasma membrane and cytoplasm, which was different from that in Arabidopsis. The transgenic Arabidopsis plant containing a BnaA02.NIP6;1a promoter driven GUS reporter gene displayed strong GUS activity in roots, stems, leaves, especially in buds and open flowers, which are different from the expression pattern from its homologous gene in Arabidopsis. Silencing BnaA02.NIP6;1a repressed vegetative growth under B-deficient condition in Brassica napus. More importantly, knockdown of BnaA02.NIP6;1a in rapeseed resulted in the reduction of boron accumulation in the flower under boron deficiency and lead to severe sterility, which has not yet been reported before. Furthermore, nip6;1 mutant in Arabidopsis only showed the loss of apical dominance phenotype under boron deficiency at reproductive stage, whereas BnaA02.NIP6;1 RNAi lines exhibited large amounts of abnormal development of the inflorescence as compared with the wild type under boron limitation. Taken together, our results demonstrate that BnaA02.NIP6;1a encodes a boron transporter required for plant development under boron deficiency in Brassica napus, which shows its novel and diverse function in rapeseed compared with model plant Arabidopsis.