Bioinformatics and Functional Analyses Implicate Potential Roles for EOGT and L-fringe in Pancreatic Cancers
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Updated: Nov 18, 2025
Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line
Published on: April 11, 2025
Rashu Barua1, Kazuyuki Mizuno2, Yuko Tashima1,3
1Department of Molecular Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan.
Abstract:
Notch signaling receptors, ligands, and their downstream target genes are dysregulated in pancreatic ductal adenocarcinoma (PDAC), suggesting a role of Notch signaling in pancreatic tumor development and progression. However, dysregulation of Notch signaling by post-translational modification of Notch receptors remains poorly understood. Here, we analyzed the Notch-modifying glycosyltransferase involved in the regulation of the ligand-dependent Notch signaling pathway. Bioinformatic analysis revealed that the expression of epidermal growth factor (EGF) domain-specific O-linked N-acetylglucosamine (EOGT) and Lunatic fringe (LFNG) positively correlates with a subset of Notch signaling genes in PDAC. The lack of EOGT or LFNG expression inhibited the proliferation and migration of Panc-1 cells, as observed by the inhibition of Notch activation. EOGT expression is significantly increased in the basal subtype, and low expression of both EOGT and LFNG predicts better overall survival in PDAC patients. These results imply potential roles for EOGT- and LFNG-dependent Notch signaling in PDAC.
Insights
Notch signaling is altered in pancreatic cancer. Epidermal growth factor (EGF) domain-specific O-linked N-acetylglucosamine (EOGT) and Lunatic fringe (LFNG) influence tumor growth and may predict patient survival in pancreatic ductal adenocarcinoma (PDAC).
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Notch signaling pathway components are dysregulated in pancreatic ductal adenocarcinoma (PDAC).
- Post-translational modification of Notch receptors in PDAC remains poorly understood.
- The role of Notch-modifying glycosyltransferases in PDAC is unclear.
Purpose of the Study:
- To investigate the role of the Notch-modifying glycosyltransferase EOGT and LFNG in PDAC.
- To analyze the correlation between EOGT, LFNG, and Notch signaling genes in PDAC.
- To determine the prognostic significance of EOGT and LFNG in PDAC patients.
Main Methods:
- Bioinformatic analysis of gene expression data in PDAC.
- In vitro studies using Panc-1 cells to assess the impact of EOGT and LFNG.
- Correlation analysis between EOGT/LFNG expression and Notch signaling pathway genes.
- Survival analysis in PDAC patient cohorts.
Main Results:
- EOGT and LFNG expression positively correlates with a subset of Notch signaling genes in PDAC.
- Inhibition of EOGT or LFNG reduced Panc-1 cell proliferation and migration by blocking Notch activation.
- EOGT expression is elevated in the basal subtype of PDAC.
- Low expression of both EOGT and LFNG predicts improved overall survival in PDAC patients.
Conclusions:
- EOGT and LFNG play significant roles in PDAC development and progression.
- EOGT and LFNG are potential therapeutic targets and prognostic biomarkers for PDAC.
- Further research into EOGT- and LFNG-dependent Notch signaling in PDAC is warranted.
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