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Age-dependent structural changes in human neuronal chromatin.

M Ermini, M L Moret, K Reichlmeier

    Aktuelle Gerontologie
    |December 1, 1978
    PubMed
    Summary
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    Aging increases the length of DNA segments in brain cells, affecting chromatin structure. This age-related change in DNA repeat length impacts how DNA is packaged and accessed in neurons.

    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Gerontology

    Background:

    • Chromatin structure is fundamental to DNA accessibility and gene regulation.
    • Aging is associated with cellular and molecular alterations, including changes in DNA packaging.

    Purpose of the Study:

    • To investigate age-related changes in the nucleosomal DNA repeat length and DNA digestibility in human cerebral cortex neurons.
    • To determine if alterations in chromatin structure occur with aging.

    Main Methods:

    • Extraction of DNA from neuronal nuclei of young and old human subjects.
    • Partial digestion of DNA with micrococcal nuclease.
    • Gel electrophoresis and determination of DNA fragment base-pair content.

    Main Results:

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    • Nucleosomal DNA repeat length increased from 170 bp in young to 199 bp in old individuals.
    • The increase in repeat length was attributed to the linker DNA region, with core DNA remaining constant at ~140 bp.
    • Older neurons showed reduced nuclear DNA digestibility by micrococcal nuclease compared to younger neurons.

    Conclusions:

    • Aging is associated with an increase in chromosomal DNA repeat length, indicating alterations in basic chromatin structure.
    • Changes in DNA digestibility with age may be due to modifications in chromatin structure or superstructure.