Research progress and the biotechnological applications of multienzyme complex

Area of Science:

• Synthetic biology
• Biocatalysis
• Biomanufacturing

Background:

• Multienzyme complexes are crucial in synthetic biology for efficient catalysis.
• They enable continuous substrate conversion through enzyme cascades.
• In vitro systems offer advantages over microbial cells, including higher yields and easier separation.

Purpose of the Study:

• To review natural multienzyme complexes and their cascade reactions.
• To summarize methods for constructing artificial multienzyme complexes.
• To discuss the structure and applications of cellulosomes.

Main Methods:

• Literature review of natural and artificial multienzyme complexes.
• Analysis of enzyme cascade strategies in vivo and in vitro.
• Examination of scaffolding protein roles in enzyme organization.

Main Results:

• Natural enzyme cascades exhibit high catalytic efficiency.
• Artificial multienzyme complexes can be constructed using various approaches.
• Cellulosomes represent a key example of complex multienzyme systems with diverse applications.

Conclusions:

• Multienzyme complexes provide a robust platform for synthetic biology.
• Further research can enhance multienzyme complex design and application.
• This review offers a theoretical basis for advancing multienzyme complex technology in biomanufacturing.