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An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

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Identification of the transgene insertion site for an adipocyte-specific adiponectin-cre model and characterization

Jared S Farrar¹, Joseph C Lownik¹, Grayson W Way²

¹Center for Clinical and Translational Research, Virginia Commonwealth University School of Medicine , Richmond, VA, USA.

|February 10, 2021

Abstract:

The Adipoq-Cre transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective Adipoq-Cre transgenic mouse, strong genetic linkage between a gene of interest, Adam10, and the Adipoq-Cre transgene was discovered. Whole-genome sequencing of the Adipoq-Cre transgenic mouse model identified the genomic insertion site within the Tbx18 gene locus on chromosome 9 and this insertion causes a significant decrease in Tbx18 gene expression in adipose tissue. Insertion of genes Kng2, Kng1, Eif4a2 and Rfc4 also occurred in the Adipoq-Cre transgenic mouse, and these passenger genes may have functional consequences in various tissues.

Keywords:

Adipoq-cre adipocyte bacterial artificial chromosome insertion site passenger genes

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