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Subcellular Localization of uc.8+ as a Prognostic Biomarker in Bladder Cancer Tissue.

Sara Terreri1,2, Sara Mancinelli1,3,4, Matteo Ferro5

  • 1Institute of Genetics and Biophysics "A. Buzzati Traverso", National Research Council (CNR), 80131 Naples, Italy.

Cancers
|February 11, 2021
PubMed
Summary

Ultra-conserved-transcript-8+ (uc.8+) shows distinct expression and localization patterns in bladder cancer. Its subcellular localization may serve as a novel prognostic biomarker for bladder cancer patients.

Keywords:
bladder cancerlong noncoding RNAprognostic biomarkertranscribed-ultraconserved regionultraconserved region

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-coding RNAs from Ultraconserved Regions (UCRs) are implicated in cancer pathophysiology.
  • Ultra-conserved-transcript-8+ (uc.8+) has shown correlation with bladder cancer grading and staging.

Purpose of the Study:

  • To validate uc.8+ as a potential biomarker in bladder cancer.
  • To assess uc.8+ expression and subcellular localization in human bladder cancer specimens.

Main Methods:

  • Tissue microarray analysis of 73 human bladder cancer specimens.
  • In-situ hybridization for uc.8+ quantification.
  • Correlation analysis with clinical characteristics and patient survival.
  • Deep machine-learning for sequence and RNA binding protein identification.

Main Results:

  • uc.8+ exhibited distinct subcellular localization: nucleus and cytoplasm in low-grade, predominantly cytoplasm in high-grade tumors, suggesting nuclear-to-cytoplasmic translocation.
  • Down-regulation of uc.8+ was observed in tumor-surrounding stroma compared to tumor regions.
  • Machine learning identified sequences linked to uc.8+ localization and potential mRNA translocation factors.

Conclusions:

  • uc.8+ subcellular localization is a potential prognostic biomarker for bladder cancer.
  • The observed translocation patterns may reflect tumor progression.
  • Further research into uc.8+ and its interacting proteins could reveal new therapeutic targets.