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Interactions between ScNAC23 and ScGAI regulate GA-mediated flowering and senescence in sugarcane
Jinlan Fang1, Zhe Chai1, Wei Yao2
1State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi University, Nanning, 530005, China; Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning, 530005, China.
Plant Science : an International Journal of Experimental Plant Biology
|February 11, 2021
Summary
Researchers identified a sugarcane NAC transcription factor (ScNAC23) that accelerates plant flowering and leaf senescence. This factor interacts with gibberellin (GA) signaling, revealing a new regulatory module for plant development.
Area of Science:
- Plant Molecular Biology
- Plant Physiology
- Gene Regulation
Background:
- Gene transcription control is vital for plant growth, development, flowering, leaf senescence, and seed germination.
- Sugarcane (Saccharum officinarum) development is influenced by various genetic and hormonal factors.
- Gibberellin (GA) is a key plant hormone regulating numerous growth and developmental processes.
Purpose of the Study:
- To identify and characterize a novel NAC transcription factor from sugarcane involved in plant development.
- To investigate the role of the identified NAC transcription factor in regulating flowering and leaf senescence.
- To elucidate the interaction between the NAC transcription factor and gibberellin signaling pathways in sugarcane.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) was used to analyze ScNAC23 gene expression.
- Ectopic expression of ScNAC23 in Arabidopsis thaliana was performed to assess its function.
- Yeast-two hybrid, bimolecular fluorescence complementation, and GST-pull down assays confirmed protein interactions.
Main Results:
- ScNAC23 expression was induced in mature sugarcane leaves and flowering varieties, and responsive to GA.
- Overexpression of ScNAC23 in Arabidopsis accelerated flowering and leaf senescence and increased sensitivity to GA.
- ScNAC23 directly interacts with ScGAI, an inhibitor of GA signaling, suggesting a regulatory link.
Conclusions:
- ScNAC23 is a sugarcane NAC transcription factor that promotes flowering and leaf senescence.
- A regulatory module involving GA, ScNAC23, and downstream targets like LFY/SAGs is proposed.
- This finding offers new insights into the molecular mechanisms by which GA accelerates flowering and senescence in sugarcane.
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