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MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
Agnieszka Piasecka1, Michał Sekrecki1, Michał Wojciech Szcześniak2
1Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznanskiego 6, 61-614, Poznań, Poland.
Scientific Reports
|February 11, 2021
Summary
Myocyte enhancer factor 2C (MEF2C) regulates muscle cell growth and differentiation. This study reveals MEF2C impacts microRNA quantity and quality, affecting muscle development.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- Myocyte enhancer factor 2C (MEF2C) is a key transcription factor in muscle development.
- Its role in microRNA regulation during myogenesis is largely unknown.
Purpose of the Study:
- To investigate MEF2C's contribution to microRNA composition and dynamics in muscle differentiation.
- To understand how MEF2C influences microRNA quantity and quality.
Main Methods:
- Deep sequencing of small RNAs from human muscle cells with MEF2C depletion.
- Analysis of microRNA expression, transcription efficiency, and 3'-end modifications.
Main Results:
- MEF2C depletion alters a significant set of microRNAs, with both down- and up-regulation observed.
- MEF2C influences microRNA transcription efficiency and 3'-end oligouridylation mediated by TUT4.
- A quarter of differentiation-responsive miRNAs show inverse changes in MEF2C-deficient cells.
Conclusions:
- MEF2C is crucial for regulating microRNA quantity and quality during muscle cell differentiation.
- MEF2C affects microRNA stability and specificity, impacting myogenic programs.
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