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LncRNA MCTP1-AS1 Regulates EMT Process in Endometrial Cancer by Targeting the miR-650/SMAD7 Axis

Qin Gao1, Qin Huang1, Fangbing Li1

  • 1Obstetrics and Gynecology of Pu Ren Hospital in Wuhan, Wuhan, 430081, People's Republic of China.

Oncotargets and Therapy
|February 11, 2021
PubMed
Abstract

Insights

Long noncoding RNA MTCP1-AS1 is downregulated in endometrial cancer (EC). Upregulating MTCP1-AS1 inhibits EC cell growth, migration, and invasion by targeting the miR-650/SMAD7 pathway, suggesting its therapeutic potential.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various diseases, including cancer.
  • Investigating specific lncRNAs like MTCP1-AS1 is crucial for understanding cancer pathogenesis.

Purpose of the Study:

  • To examine the expression of MTCP1-AS1 in endometrial cancer (EC).
  • To elucidate the functional role of MTCP1-AS1 in EC cell behavior and its underlying molecular mechanisms.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for expression analysis.
  • Cell proliferation (CCK8), migration (wound-healing), invasion (Transwell), and epithelial-to-mesenchymal transition (EMT) assays.
  • Luciferase reporter and RNA-binding protein immunoprecipitation (RIP) assays to confirm molecular interactions.

Main Results:

  • MTCP1-AS1 expression was significantly downregulated in EC tissues and cell lines.
  • Overexpression of MTCP1-AS1 suppressed EC cell proliferation, migration, invasion, and EMT.
  • MTCP1-AS1 acts as a target of miR-650, negatively regulating its expression and influencing the miR-650/SMAD7 axis.

Conclusions:

  • lncRNA MTCP1-AS1 inhibits EC cell proliferation, migration, invasion, and EMT by targeting the miR-650/SMAD7 axis.
  • MTCP1-AS1 demonstrates potential as a novel therapeutic target for endometrial cancer.
Keywords:
ECEMTMCTP1-AS1SMAD7endometrial cancerlncRNAsmiR-650

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