Related Experiment Video

Updated: Nov 17, 2025

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

6.8K

Hsa_circ_0043278 Inhibits Tumorigenesis and is Downregulated in Colorectal Cancer

Jiali Wang1,2, Tiangong Wang2, Shiyun Hu2

  • 1Department of Oncology and Hematology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, People's Republic of China.

Cancer Management and Research
|February 11, 2021
PubMed
Abstract

Insights

Circular RNA hsa_circ_0043278 is downregulated in colorectal cancer, inhibiting tumor growth. This finding suggests its potential as a diagnostic biomarker and therapeutic target for colorectal cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are novel endogenous RNAs implicated in tumorigenesis.
  • The specific roles and diagnostic value of most circRNAs in colorectal cancer remain largely unknown.

Purpose of the Study:

  • To investigate the expression and function of circRNAs in colorectal cancer.
  • To evaluate the potential of circRNAs as diagnostic biomarkers for colorectal cancer.

Main Methods:

  • Bioinformatic analysis of circRNA expression profiles in colorectal cancer patients.
  • Quantitative real-time polymerase chain reaction to measure hsa_circ_0043278 expression in tumor tissues and cell lines.
  • Functional assays (Cell Counting Kit-8, colony formation, Transwell, flow cytometry) to assess the impact of hsa_circ_0043278 on cell proliferation, migration, and apoptosis.

Main Results:

  • hsa_circ_0043278 was significantly downregulated in colorectal cancer tissues and cell lines.
  • hsa_circ_0043278 demonstrated diagnostic potential with an area under the receiver operating characteristic curve of 0.71.
  • Overexpression of hsa_circ_0043278 suppressed colorectal cancer cell proliferation and migration while promoting apoptosis.

Conclusions:

  • hsa_circ_0043278 acts as a tumor suppressor in colorectal cancer.
  • hsa_circ_0043278 is a promising biomarker for colorectal cancer diagnosis.
  • hsa_circ_0043278 may serve as a potential therapeutic target for colorectal cancer.
Keywords:
apoptosiscircular RNAsmolecular markersuppressortumorigenesis

More Related Videos

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

5.8K
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

787

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
8.0K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.5K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.6K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.3K