Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.2K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.2K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.1K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.1K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.6K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.6K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

15.2K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.2K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

8.6K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impaired Fine Motor Skills in Developmental Dyslexia: A Bayesian Meta-Analysis of 5 Decades of Research.

Journal of speech, language, and hearing research : JSLHR·2026
Same author

Transient gingival inflammation is associated with epithelial dysfunction and systemic immune activation beyond clinical improvement.

Journal of periodontology·2026
Same author

Medication-related osteonecrosis of the jaw: incidence and risk factors in multiple myeloma patients undergoing pre-autologous stem cell transplant dental extractions.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

Phosphorylated lantibiotics-producing commensals integrate into the human oral microbiome to suppress pathogens and promote microbiome homeostasis.

NPJ biofilms and microbiomes·2026
Same author

Machine Learning Models for Individualized Osteoradionecrosis Risk Prediction in Head and Neck Cancer.

Journal of medical systems·2026
Same author

A Bayesian Meta-Analysis of Lexical Priming and Reaction Time in Children With and Without Developmental Language Disorder.

Journal of speech, language, and hearing research : JSLHR·2026

Related Experiment Video

Updated: Nov 17, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.2K

CD301 mediates fusion in IL-4-driven multinucleated giant cell formation.

Patricia J Brooks1,2, Yongqiang Wang1, Marco A Magalhaes1

  • 1Faculty of Dentistry, University of Toronto, Toronto, Ontario M5G 1G6, Canada.

Journal of Cell Science
|February 11, 2021
PubMed
Summary

Interleukin-4 (IL-4) signaling upregulates C-type lectin domain family 10 member (CD301) in macrophages, promoting multinucleated giant cell (MGC) formation through enhanced cell adhesion and fusion.

Keywords:
Foreign body giant cellsIL-4InflammationMacrophageMass spectrometryMonocyte

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.8K
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
08:01

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

12.7K

Related Experiment Videos

Last Updated: Nov 17, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.2K
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.8K
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
08:01

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

12.7K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Multinucleated giant cells (MGCs) are critical in various inflammatory and immune responses.
  • The precise molecular mechanisms driving MGC formation remain incompletely understood.
  • Identifying key regulators of MGC development is crucial for understanding disease pathogenesis.

Purpose of the Study:

  • To identify novel molecular determinants involved in interleukin-4 (IL-4)-induced MGC formation.
  • To investigate the role of C-type lectin domain family 10 member (CD301) in MGC development.
  • To elucidate the function of CD301 in mediating cell adhesion and fusion.

Main Methods:

  • Tandem mass tag-mass spectrometry (TMT-MS) for proteomic analysis of macrophages.
  • Interleukin-4 (IL-4) stimulation of mouse RAW264.7 and primary murine macrophages.
  • CRISPR/Cas9 gene editing to delete CD301 genes (Mgl1 and Mgl2).
  • Functional assays using N-acetylgalactosamine-coated beads to assess cell binding.
  • Immunohistochemical analysis of human inflammatory lesions.

Main Results:

  • CD301 (CLEC10A) was identified as significantly upregulated in IL-4-stimulated macrophages undergoing MGC formation.
  • CD301 was detected in MGCs within human inflammatory biopsy specimens.
  • Antibody-mediated inhibition or genetic deletion of CD301 significantly reduced IL-4-induced cell binding and MGC formation.
  • Re-expression of CD301 restored MGC formation in knockout cells.

Conclusions:

  • CD301 plays a critical role in IL-4-driven MGC formation.
  • CD301 mediates intercellular adhesion and fusion, essential processes for MGC development.
  • This study identifies CD301 as a key molecular determinant in MGC biology.