A novel fluorescent probe for rapid detection of sulfur dioxide in living cells

Sulfur dioxide is one of the reactive sulfur species, which has significant physiological functions in cells. Some physiological processes are closely related to SO₂ in organisms, and the high concentration of SO₂ in living cells can cause many diseases. In order to investigate the unique function of SO₂ at the subcellular level, developing a molecular tool which could detect of SO₂ within organelles is imperative. Hence, we developed a cationic dye named HQ-SO₂ as a new fluorescent probe to specifically monitor SO₂ , which was easy to obtain through one-step reaction. It took Michael addition reaction as the mechanism of reaction for detection of SO₂ . In addition, this probe showed a series of highly favorable properties such as rapid response rate, low cytotoxicity, high selectivity, low detection limit, and good photostability, which enabled the probe to track SO₂ in living cells.