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Related Experiment Video

Updated: Nov 17, 2025

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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Lentiviral Vector Bioprocessing.

Christopher Perry1,2, Andrea C M E Rayat1

  • 1The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Gower St, London WC1E 6BT, UK.

Viruses
|February 12, 2021
PubMed
Summary
This summary is machine-generated.

Lentiviral vectors (LVs) are crucial for gene therapy, enabling treatments for genetic diseases and CAR-T therapies. This review details LV bioprocess operations, focusing on scalable production for clinical applications.

Keywords:
Lentiviral vectorsbioprocessingcell and gene therapylentivirusmanufacturingpseudotyping

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Area of Science:

  • Biotechnology and Bioprocessing
  • Gene Therapy Vector Production

Background:

  • Lentiviral vectors (LVs) are essential tools for gene delivery in cell and gene therapy, including treatments for monogenic diseases and CAR-T therapies.
  • Optimizing LV production is critical for advancing clinical applications and ensuring therapeutic efficacy.

Purpose of the Study:

  • To provide a comprehensive review of individual bioprocess operations for lentiviral vector production.
  • To highlight opportunities for bioprocess improvement and optimization for scalable LV manufacturing.
  • To discuss considerations for achieving high-quality, high-concentration lentiviral vectors.

Main Methods:

  • Review of upstream culture methods, including transfection techniques and bioreactor choices for producer cell cultivation.
  • Evaluation of downstream purification strategies, focusing on chromatography and tangential flow filtration for scalable production.
  • Summary of vector quantification and characterization assays.

Main Results:

  • Envelope proteins play a significant role in vector design and bioprocessing efficiency.
  • Different sequences of downstream operations are suitable for both small- and large-scale LV production.
  • Tangential flow filtration is a key technology for scalable LV purification.

Conclusions:

  • Understanding the interactions between different bioprocess steps is crucial for robust and scalable LV production.
  • This review provides insights to achieve high-quality, high-concentration lentiviral vectors through optimized bioprocessing.
  • Continuous improvement in LV bioprocessing is essential for the advancement of cell and gene therapies.