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Ricin Antibodies' Neutralizing Capacity against Different Ricin Isoforms and Cultivars.

Maria Lucia Orsini Delgado1, Arnaud Avril2, Julie Prigent1

  • 1Paris-Saclay University, CEA, INRAE, Medicines and Healthcare Technologies Department (DMTS), SPI, 91191 Gif-sur-Yvette, France.

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|February 12, 2021
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Summary

Antibodies targeting ricin toxin show promise for biodefense. Combining anti-ricin A-chain and anti-ricin B-chain antibodies offers synergistic protection against ricin isoforms in mice.

Keywords:
Ricinus communis cultivarsmonoclonal antibodiesmouse modelneutralizing antibodiespulmonary intoxicationrecombinant antibodiesricinricin isoformstreatment

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Area of Science:

  • Biochemistry
  • Immunology
  • Toxicology

Background:

  • Ricin, a potent toxin from castor beans, is a biowarfare concern.
  • No approved treatments exist for ricin intoxication.
  • Antibodies offer immediate protection but may lack broad-spectrum efficacy against ricin variants.

Purpose of the Study:

  • To evaluate the protective capacity of specific antibodies against different ricin isoforms (D and E).
  • To investigate the potential for synergistic effects between different anti-ricin antibodies.
  • To assess the in vivo efficacy of antibody combinations in a mouse model.

Main Methods:

  • Characterization of antibody binding to ricin isoforms D and E.
  • In vitro assessment of neutralizing capacity against different ricin cultivars.
  • In vivo intranasal ricin intoxication model in mice with post-exposure antibody treatment.

Main Results:

  • Antibodies RB34 and RB37 recognized only ricin D; 43RCA-G1 recognized both isoforms.
  • In vitro neutralization varied by ricin cultivar.
  • A synergistic protective effect was observed when combining RB34 and 43RCA-G1 antibodies.
  • In vivo studies showed significant protection in mice treated with the antibody combination post-intoxication.

Conclusions:

  • Antibody efficacy against ricin is isoform-dependent.
  • Combining antibodies targeting different ricin components enhances protective capacity.
  • Broad-spectrum evaluation of antibodies is crucial for developing effective ricin countermeasures.