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Related Experiment Videos

Paired sequence difference in ribosomal RNAs: evolutionary and phylogenetic implications.

W C Wheeler1, R L Honeycutt

  • 1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138.

Molecular Biology and Evolution
|January 1, 1988
PubMed
Summary

Darwinian selection maintains the secondary structure of ribosomal RNAs (rRNAs). Analysis of 5S rRNA sequences reveals that functionally important pairings influence insect phylogenies, differing from non-pairing regions.

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Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Bioinformatics

Background:

  • Ribosomal RNAs (rRNAs) possess crucial secondary structures stabilized by Watson-Crick base pairing.
  • Understanding the evolutionary pressures on rRNA sequences is vital for phylogenetic analysis.

Purpose of the Study:

  • To investigate the role of Darwinian selection in maintaining rRNA secondary structure.
  • To assess the impact of structure-preserving nucleotide positions on phylogenetic reconstruction.

Main Methods:

  • Comparative sequence analysis of chordate, arthropod, and plant 5S ribosomal RNA (rRNA).
  • Phylogenetic tree construction using nucleotide positions involved in secondary structure pairing versus non-pairing positions.

Main Results:

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  • Evidence suggests Darwinian selection actively maintains functionally important secondary structures in 5S rRNA sequences.
  • Insect phylogenies derived from pairing-related nucleotide positions are incongruent with those from non-pairing positions.
  • Phylogenetic reconstructions using non-pairing bases align with established morphological data.

Conclusions:

  • Nucleotide sequences in rRNAs are under selective pressure to maintain secondary structure.
  • Phylogenetic signals derived from structure-forming versus non-structure-forming regions can differ significantly.
  • Morphological data supports phylogenies inferred from non-pairing rRNA bases, highlighting the impact of structural constraints on sequence evolution.