Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

38.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
38.7K
The DNA Replication Fork01:02

The DNA Replication Fork

17.2K
17.2K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.8K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.8K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.1K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.7K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.7K
DNA Damage Can Stall the Cell Cycle02:37

DNA Damage Can Stall the Cell Cycle

2.9K
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic Potential of Extracellular Vesicles Derived from Sheep Placenta in the Treatment of Skin Disorders.

ACS omega·2026
Same author

[Study on mechanism of serum transitional components of Yinxing Yangnao Formula against vascular dementia based on UPLC- Orbitrap-HRMS combined with network pharmacology].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2026
Same author

Beyond Terminal Blockade: A Mechanism-Based Approach to Complement Inhibitor Selection in Paroxysmal Nocturnal Hemoglobinuria.

Drug design, development and therapy·2026
Same author

A preliminary study on the combined assessment of 25-hydroxyvitamin D and thyroid function for predicting diabetic foot risk and amputation in type 2 diabetes.

Frontiers in endocrinology·2026
Same author

Low-Temperature Deposition of Polycrystalline ε-Ga<sub>2</sub>O<sub>3</sub> for Deep Ultraviolet Perceptual Photodetection.

The journal of physical chemistry letters·2026
Same author

Genomic analysis of <i>Botrytis cinerea</i> causing postharvest strawberry rot and the control effect of pydiflumetofen.

Frontiers in microbiology·2026

Related Experiment Video

Updated: Nov 17, 2025

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.3K

A novel WEE1 pathway for replication stress responses.

Ting Pan1, Qi Qin1, Chubing Nong1

  • 1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.

Nature Plants
|February 12, 2021
PubMed
Summary

Scientists discovered a new WEE1 protein pathway that helps cells respond to DNA replication stress. This pathway involves FBL17/SKP2, leading to CDK inhibitor accumulation and CDK inhibition, crucial for genome stability and cancer therapy.

More Related Videos

Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity
10:12

Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity

Published on: June 14, 2024

2.3K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.5K

Related Experiment Videos

Last Updated: Nov 17, 2025

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.3K
Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity
10:12

Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity

Published on: June 14, 2024

2.3K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.5K

Area of Science:

  • Molecular Biology
  • Plant Science
  • Cancer Research

Background:

  • DNA replication stress threatens genome stability and is a cancer hallmark.
  • WEE1 kinase is known to regulate replication stress by inhibiting CDKs.

Purpose of the Study:

  • To elucidate a novel WEE1-mediated pathway in response to replication stress.
  • To investigate the role of FBL17 in WEE1 signaling.

Main Methods:

  • Investigated protein-protein interactions between WEE1 and FBL17 in Arabidopsis.
  • Analyzed phosphorylation and ubiquitination of FBL17.
  • Assessed the impact of FBL17 loss-of-function and CDK inhibitor overexpression on wee1 mutant phenotypes.
  • Examined the homologous interaction in human cells.

Main Results:

  • Arabidopsis WEE1 directly interacts with and phosphorylates E3 ligase FBL17.
  • Phosphorylated FBL17 undergoes polyubiquitination and degradation, increasing CDK inhibitors.
  • Loss of FBL17 or increased CDK inhibitors rescues wee1 mutant replication stress sensitivity.
  • Human WEE1 phosphorylates and destabilizes SKP2, a FBL17 homolog.

Conclusions:

  • Identified the conserved WEE1-FBL17/SKP2-CKIs-CDKs axis as a key replication stress response pathway.
  • This pathway regulates CDK activity through targeted degradation of inhibitors.
  • The findings have potential clinical implications for WEE1 inhibitor-based cancer therapy.