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Increased Expression of DNA2 Was Linked to Poor Prognosis in Breast Cancer

Yingyan Han1, Zeyu Zhang1, Zhi Wang1

  • 1Cancer Biology Research Center (Key Laboratory of the Ministry of Education), Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Disease Markers
|February 12, 2021
PubMed

Insights

Elevated DNA2 nuclease expression correlates with poor breast cancer prognosis and promotes tumor growth. Silencing DNA2 inhibits cancer cell proliferation, identifying DNA2 as a potential diagnostic and prognostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Homologous recombination (HR) is crucial for breast cancer cell survival.
  • DNA2 nuclease functions in parallel with HR in DNA double-strand break (DSB) repair.

Purpose of the Study:

  • To investigate the clinical attributes of DNA2 in breast cancer.
  • To determine the role of DNA2 in breast cancer cell growth and progression.

Main Methods:

  • Analysis of large breast cancer datasets (cBioportal, bc-GenExMiner).
  • Immunohistochemical staining of breast cancer tissues.
  • Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO), and KEGG pathway analyses.
  • In vitro cell growth assays and in vivo tumor suppression studies.

Main Results:

  • Elevated DNA2 expression is linked to poor breast cancer prognosis.
  • DNA2 expression is increased in ER-negative, PR-negative, HER2-positive, and high-grade breast cancers.
  • DNA2 is enriched in cell cycle and DNA replication pathways.
  • Silencing DNA2 inhibits breast cancer cell growth and tumor progression.

Conclusions:

  • DNA2 is a potential prognostic biomarker for breast cancer.
  • DNA2 plays a significant role in breast cancer progression.
  • Further preclinical and clinical investigations of DNA2 are warranted.

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