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Updated: Nov 17, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
Xinxin Zhou1, Jiayuan He2, Qingyuan Wang2
1Academy of Traditional Chinese Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
Abstract:
The functions of non-coding RNA, including microRNA (miRNA), have attracted considerable attention in the field of oncology, In this report, we examined the roles and molecular mechanisms of miR-128-3p, as related to the biological behaviors of malignant melanoma (MM). We found that miR-128-3p was expressed in low levels in these MM cells and may serve as a tumor suppressor by inhibiting proliferation, migration, and invasion, as well as inducing apoptosis in these MM cells. Moreover, neurotrophin receptor 3 (NTRK3), which serves as an oncogene that can enhance malignant behaviors of MM cells, was up-regulated in MM cells. Our current survey disclosed a complementary binding between miR-128-3p and the NTRK3 3' untranslated regions (3'-UTR), while luciferase activities of NTRK3 3'-UTR were restrained by miR-128-3p in 293T cells. The effects of pre-miR-128-3p and sh-NTRK3 as well as anti-miR-128-3p and NTRK3(+) appeared to function synergistically in producing malignant progression. Moreover, there were possible to have counteracted effects for pre-miR-128-3p and NTRK3(+) in malignant progression. These findings established that miR-128-3p can function as a tumor suppressor by inhibiting carcinogenesis of the oncogene, NTRK3. Collectively, miR-128-3p and NTRK3 genes participate in modulating the malignant behavior of MM, and may represent new therapeutic targets for MM.
Insights
MicroRNA-128-3p acts as a tumor suppressor in malignant melanoma by inhibiting proliferation and invasion. It targets the oncogene neurotrophin receptor 3 (NTRK3), suggesting potential therapeutic strategies for melanoma.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Non-coding RNAs, particularly microRNAs (miRNAs), are increasingly recognized for their roles in cancer.
- Malignant melanoma (MM) is an aggressive skin cancer with complex molecular underpinnings.
- Understanding the specific functions of miRNAs in MM is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the role and molecular mechanisms of miR-128-3p in the biological behaviors of malignant melanoma.
- To determine the relationship between miR-128-3p and the expression of neurotrophin receptor 3 (NTRK3) in MM cells.
Main Methods:
- Quantitative analysis of miR-128-3p expression in MM cells.
- Assessment of cell proliferation, migration, invasion, and apoptosis.
- Luciferase reporter assays to confirm the binding of miR-128-3p to the NTRK3 3'-UTR.
- Experimental manipulation of miR-128-3p and NTRK3 levels.
Main Results:
- miR-128-3p was found to be expressed at low levels in MM cells.
- Overexpression of miR-128-3p inhibited MM cell proliferation, migration, and invasion, while promoting apoptosis.
- Neurotrophin receptor 3 (NTRK3) was upregulated in MM cells and acts as an oncogene.
- miR-128-3p directly binds to the 3'-UTR of NTRK3, suppressing its activity.
- miR-128-3p and NTRK3 exhibit synergistic and counteracting effects on malignant progression.
Conclusions:
- miR-128-3p functions as a tumor suppressor in malignant melanoma by inhibiting the oncogenic activity of NTRK3.
- The miR-128-3p/NTRK3 axis plays a significant role in modulating the malignant behavior of MM.
- Both miR-128-3p and NTRK3 represent potential therapeutic targets for MM treatment.
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