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Updated: Nov 17, 2025

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
Published on: February 10, 2023
A minimum reporting standard for multiple sequence alignments
Thomas K F Wong1,2, Subha Kalyaanamoorthy1,3, Karen Meusemann4,5,6
1Land & Water, CSIRO, Canberra, ACT 2601, Australia.
Abstract:
Multiple sequence alignments (MSAs) play a pivotal role in studies of molecular sequence data, but nobody has developed a minimum reporting standard (MRS) to quantify the completeness of MSAs in terms of completely specified nucleotides or amino acids. We present an MRS that relies on four simple completeness metrics. The metrics are implemented in AliStat, a program developed to support the MRS. A survey of published MSAs illustrates the benefits and unprecedented transparency offered by the MRS.
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