Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lagging Strand Synthesis01:59

Lagging Strand Synthesis

58.5K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
58.5K
The Replisome03:01

The Replisome

37.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.1K
Chromosome Replication02:31

Chromosome Replication

9.8K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
9.8K
The DNA Replication Fork01:02

The DNA Replication Fork

38.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
38.7K
Replication in Eukaryotes01:29

Replication in Eukaryotes

16.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
16.0K
Replication in Eukaryotes02:31

Replication in Eukaryotes

186.3K
Overview
186.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maxillo-mandibular changes after maxillary skeletal expansion according to skeletal subtype: a retrospective cohort study.

European journal of orthodontics·2026
Same author

Transverse skeletal proportionality in the prognostic evaluation of facemask therapy for skeletal class III malocclusion: a retrospective cephalometric study.

Progress in orthodontics·2026
Same author

ATR and TopBP1 oppose to control dormant origin activity and global replication dynamics, providing a first defense against replication stress.

Nucleic acids research·2026
Same author

Training biologists in Unix command-line skills: From curriculum to interactive online tutorials.

PLoS computational biology·2026
Same author

Dimensional behaviors of direct-printed versus thermoformed clear retainers in relation to changes of inter-piece occlusal contact distribution measured by T-scan after intraoral aging: a prospective clinical study.

Progress in orthodontics·2026
Same author

Proteomics and phosphoproteomics of human colorectal cancer cells lacking a specific kinase activity reveal kinase-specific compensatory responses.

Animal cells and systems·2026
Same journal

asms: finding allele-specific methylation in human genomes without phasing.

NAR genomics and bioinformatics·2026
Same journal

An epigenetic clock for chronological age estimation in East Asian populations.

NAR genomics and bioinformatics·2026
Same journal

The role of ATF4 in neurons under mitochondrial stress.

NAR genomics and bioinformatics·2026
Same journal

Distinct repeat architecture landscapes in the proteomes of protozoan parasites.

NAR genomics and bioinformatics·2026
Same journal

Long non-coding RNA triplex-dependent regulation of melanoma gene networks.

NAR genomics and bioinformatics·2026
Same journal

Challenges in predicting chromatin accessibility differences between species.

NAR genomics and bioinformatics·2026
See all related articles

Related Experiment Video

Updated: Nov 17, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.7K

Efficient, quick and easy-to-use DNA replication timing analysis with START-R suite.

Djihad Hadjadj1, Thomas Denecker2, Eva Guérin1

  • 1Pathologies de la Réplication de l'ADN, Université de Paris; Institut Jacques-Monod, UMR7592, CNRS, F-75006 Paris, France.

NAR Genomics and Bioinformatics
|February 12, 2021
PubMed
Summary
This summary is machine-generated.

We developed START-R, a user-friendly web application for analyzing DNA replication timing. This tool simplifies complex bioinformatics, enabling faster and more efficient insights into genomic organization and gene function.

More Related Videos

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.3K
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
10:17

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

8.2K

Related Experiment Videos

Last Updated: Nov 17, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.7K
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.3K
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
10:17

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

8.2K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • DNA replication timing is crucial for genome stability and cell fate.
  • Current replication timing analyses are complex, time-consuming, and require advanced bioinformatics skills.

Purpose of the Study:

  • To develop a user-friendly web application for analyzing DNA replication timing.
  • To simplify and accelerate the analysis of high-throughput replication timing data.
  • To enable biologists without extensive bioinformatics expertise to study replication timing.

Main Methods:

  • Development of the START-R (Simple Tool for the Analysis of the Replication Timing based on R) suite, an interactive web application using R.
  • Utilizing high-throughput data for DNA replication timing analysis across different organisms.
  • Automated detection of timing regions and identification of significant differences between experimental conditions.

Main Results:

  • START-R significantly reduces the time required for generating and analyzing replication timing data.
  • The application automatically detects various timing regions and identifies significant differences between conditions in approximately 15 minutes.
  • Enables efficient comparison of replication timing profiles between wild-type and mutant cell lines.

Conclusions:

  • START-R provides a quick, efficient, and accessible method for DNA replication timing analysis for all organisms.
  • This novel approach empowers biologists to investigate the impact of genes or proteins on replication and genomic organization.
  • Facilitates a deeper understanding of the interplay between replication timing, epigenomic marks, and cell fate.