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Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica.

Manli Chen1, Qing Wang1, Ya Li1

  • 1Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing, 210014, China.

Molecular Biology Reports
|February 12, 2021
PubMed
Summary

Finding stable reference genes is crucial for accurate gene expression analysis in crape myrtle (Lagerstroemia indica). This study identified optimal gene pairs for normalization across various conditions, improving qPCR reliability.

Keywords:
Abiotic stressesLagerstroemia indicaOrnamental traitsReference genesqPCR

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Area of Science:

  • Plant molecular biology
  • Gene expression analysis
  • Quantitative PCR (qPCR)

Background:

  • Accurate gene expression studies in Lagerstroemia indica (crape myrtle) are hindered by a lack of validated reference genes for normalization.
  • Reference genes are essential for normalizing quantitative gene expression data obtained through qPCR.

Purpose of the Study:

  • To identify and validate stable endogenous reference genes for quantitative gene expression analysis in Lagerstroemia indica.
  • To assess the expression stability of nine candidate reference genes across diverse experimental conditions.

Main Methods:

  • Evaluated stability of nine candidate reference genes using four statistical algorithms.
  • Tested genes across six datasets: different tissues, leaf colors, flower colors, and abiotic stresses (salt, drought, cold).
  • Verified reference gene accuracy using a target gene, LiMYB56.

Main Results:

  • The combination of two reference genes provided more accurate normalization than a single gene.
  • Optimal reference gene pairs varied by experimental condition (e.g., LiEF1α-2 + LiEF1α-3 for tissues and abiotic stress).
  • LiEF1α-2 demonstrated high expression stability across all tested conditions.

Conclusions:

  • Validated reference gene pairs enhance the accuracy of qPCR in Lagerstroemia indica.
  • LiEF1α-2 is a highly stable reference gene suitable for broad application in crape myrtle gene expression studies.
  • These findings provide essential tools for future molecular research in Lagerstroemia indica.