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Determination of Total Lipid and Lipid Classes in Marine Samples
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FISH Variants.

Nuno M Guimarães1, Nuno F Azevedo2, Carina Almeida2,3,4

  • 1LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal. nguimaraes@fe.up.pt.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2021
PubMed
Summary
This summary is machine-generated.

Fluorescence in situ hybridization (FISH) is crucial for detecting microorganisms. Advances have led to new FISH variants offering deeper insights into microbial populations, gene expression, and cell structures.

Keywords:
CARD-FISHCLASI-FISHDOPE-FISHFISHFISH-NanoSIMSFlow-FISHGene-FISHLeaf-FISHMAR-FISHMicrofluidic-FISHNAM-FISHPhage-FISHQD-FISH

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Fluorescence in situ hybridization (FISH) is a vital technique in microbiology for identifying and localizing microorganisms in complex samples.
  • The utility of FISH has significantly expanded due to advancements in signal amplification, imaging, automation, and overall method robustness.
  • These improvements have enhanced the versatility of FISH, enabling deeper investigations into microbial communities.

Purpose of the Study:

  • To review the significant advancements and variants of FISH.
  • To discuss the applications of these FISH variants in addressing limitations of classical FISH.
  • To highlight how new FISH techniques provide access to complex population composition, metabolic activity, and genetic information.

Main Methods:

  • Review of relevant literature on FISH techniques and their applications.
  • Categorization of FISH variants based on their capabilities and intended use.
  • Analysis of how specific FISH variants overcome challenges associated with traditional FISH methods.

Main Results:

  • Identification of key FISH variants that have emerged due to technological progress.
  • Demonstration of how these variants provide enhanced capabilities beyond simple detection.
  • Examples of FISH variants enabling the study of microbial population dynamics, gene expression, and subcellular localization.

Conclusions:

  • FISH technology has evolved significantly, offering powerful tools for microbial research.
  • Various FISH variants provide researchers with advanced methods to explore microbial systems in unprecedented detail.
  • The continued development of FISH promises further breakthroughs in understanding microbial life.