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FISH in Suspension or in Adherent Cells.
Francesca Di Pippo1, Diogo Queirós2, Joana Pereira2
1Water Research Institute C.N.R., Monterotondo, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2021
Summary
Fluorescence in situ hybridization (FISH) detects and counts microorganisms using DNA probes targeting rRNA. This method is detailed for analyzing mixed microbial communities, especially in environmental samples.
Area of Science:
- Microbiology
- Molecular Biology
- Environmental Science
Background:
- Fluorescence in situ hybridization (FISH) is a powerful molecular technique.
- FISH utilizes oligonucleotide DNA probes targeting ribosomal RNA (rRNA) for microbial identification.
- Accurate detection and enumeration of microorganisms are crucial in various scientific fields.
Purpose of the Study:
- To describe the protocol for applying FISH to microbial communities.
- To detail FISH methodology for both aggregated and suspended cells.
- To emphasize the application of FISH in environmental sample analysis.
Main Methods:
- Utilizing short-length oligonucleotide DNA probes.
- Probes are complementary to 16S or 23S rRNA sequences.
- Application to diverse sample types, including mixed microbial communities.
Main Results:
- FISH enables specific detection of microorganisms.
- Enumeration of microbial populations is achievable.
- The protocol is adaptable for various sample matrices.
Conclusions:
- FISH is a versatile method for microbial detection and quantification.
- The described protocol facilitates the study of complex microbial ecosystems.
- Environmental microbiology research benefits significantly from this FISH application.
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