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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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FISH in Food Samples.

Rui Rocha1,2, Carina Almeida3,4,5, Nuno F Azevedo5

  • 1CISAS - Centre for Research and Development in Agrifood Systems and Sustainability, Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Viana do Castelo, Portugal. rru@ipvc.pt.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2021
PubMed
Summary
This summary is machine-generated.

Faster detection of foodborne pathogens is crucial for public health. Fluorescence in situ Hybridization (FISH) offers a rapid alternative to traditional methods, meeting modern food industry needs for timely pathogen screening.

Keywords:
DetectionFoodborneMicroscopyPathogenPre-enrichment

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Area of Science:

  • Microbiology
  • Food Safety
  • Public Health

Background:

  • Foodborne diseases pose a significant global health risk.
  • Traditional culture plating methods are too slow for the modern food industry.
  • Rapid detection of foodborne pathogens is essential for effective control.

Purpose of the Study:

  • To provide an overview of Fluorescence in situ Hybridization (FISH) methodologies for pathogen detection in food.
  • To highlight key methodological aspects of sample preparation and analysis using FISH.
  • To present FISH as a viable, rapid alternative to conventional methods.

Main Methods:

  • Overview of Fluorescence in situ Hybridization (FISH) techniques.
  • Discussion of sample preparation protocols for food matrices.
  • Explanation of sample analysis procedures for pathogen identification.

Main Results:

  • FISH methods are a valid and faster alternative to standard plating techniques.
  • FISH is compatible with the rapid screening requirements of the food industry.
  • Methodological considerations for sample prep and analysis are crucial for FISH efficacy.

Conclusions:

  • FISH offers a rapid and reliable method for foodborne pathogen detection.
  • The adoption of FISH can enhance food safety protocols.
  • Optimized sample preparation and analysis are key to successful FISH implementation.