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Assessment of plasma microRNA potentials as a non-invasive biomarker in patients with axial spondyloarthropathy
T Yildirim1, E Yesilada, F Eren
1Inonu University, Faculty of Medicine, Department of Physical Medicine and Rehabilitation, Malatya, Turkey. drtulayoner@hotmail.com.
Objective:
It is assumed that abnormally expressed MicroRNAs (miRNAs) may be present in the plasma of patients with radiographic axial spondyloarthropathy (rad-AxSpA). Thus, the present study was conducted with the aim of investigating the expression profile of miRNAs in patients with rad-AxSpA.
Patients And Methods:
A total of 15 patients diagnosed with rad-AxSpA according to the Assessment of the SpondyloArthritis International Society (ASAS) classification criteria and nine healthy controls matched for age and gender were included in the study. Demographic data were collected, and disease activity was evaluated using the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Peripheral blood samples were collected, and miRNAs were extracted. The expression of microRNAs was analyzed using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) by the miScript miRNA PCR Array Human Inflammatory Response and Autoimmunity.
Results:
A total of 84 miRNA profiles were evaluated, and expressions in the study and control groups were compared. When compared to the control group, 6 miRNAs (miR-125b-5p, miR-144-3p, miR-19a-3p, miR-20a-5p, miR-29c-3p, miR-30b-5p) were detected to be upregulated, and 42 miRNAs were detected to be downregulated in the rad-AxSpA group. A p-value < 0.05 was accepted as statistically significant. A significant association was found between miR-145-5p and BASDAI (p = 0.04941). MiR-144-3p, miR-302b-3p, miR-381-3p, miR-497-5p, miR-511-5p, and miR-9-5p were found to be significantly upregulated in the HLA-B27+ patients (p = 0.03063).
Conclusions:
Abnormal miRNA expressions were detected in the plasma of the patients with rad-AxSpA. It was concluded that comprehensive studies should be continued to define these miRNAs as diagnostic biomarkers for rad-AxSpA in order to detect its association with Ankylosing Spondylitis disease activity.
Insights
Abnormal microRNA (miRNA) expressions were found in patients with radiographic axial spondyloarthropathy (rad-AxSpA). These findings suggest potential diagnostic biomarkers for rad-AxSpA and its association with disease activity.
Area of Science:
- Biochemistry
- Genetics
- Immunology
Background:
- Radiographic axial spondyloarthropathy (rad-AxSpA) is a chronic inflammatory condition.
- MicroRNAs (miRNAs) are small non-coding RNAs involved in gene regulation.
- Altered miRNA expression is implicated in various diseases, including inflammatory conditions.
Purpose of the Study:
- To investigate the expression profile of miRNAs in the plasma of patients with rad-AxSpA.
- To identify potential miRNA biomarkers for rad-AxSpA diagnosis.
- To explore the association between miRNA expression and disease activity in rad-AxSpA.
Main Methods:
- 15 patients with rad-AxSpA and 9 healthy controls were included.
- Plasma samples were collected, and miRNA expression was analyzed using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR).
- 84 miRNA profiles were evaluated, comparing rad-AxSpA patients to controls.
Main Results:
- 6 miRNAs were upregulated and 42 were downregulated in rad-AxSpA patients compared to controls.
- A significant association was found between miR-145-5p and disease activity (BASDAI).
- Several miRNAs were significantly upregulated in HLA-B27 positive patients.
Conclusions:
- Abnormal miRNA expression is present in the plasma of rad-AxSpA patients.
- Further studies are needed to validate these miRNAs as diagnostic biomarkers for rad-AxSpA.
- Investigating these miRNAs may help understand rad-AxSpA pathogenesis and disease activity.
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